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MultiTag Protein: Multiple-Epitope Tagging Loading Control Proteins

Epitope tagging is a technique in which a known epitope is fused to a recombinant protein by means of genetic engineering. By choosing an epitope for which an antibody is available, the technique renders it possible to detect proteins for which no antibody is available. This is especially useful for the characterization of newly discovered proteins and proteins of low immunogenicity.

By selection of the appropriate epitope and antibody, it is possible to find a combination with properties suitable to the desired experimental application, such as Western blot analysis, immunoprecipitation, immunochemistry, or affinity purification.

  • Never fail at Western blotting. You control the Western results
  • See positive results for every Western run. Use only 50 ng proteins as controls
  • Save time and resources for all your protein research projects

MultiTag Protein Amino Acid Sequence

MultiTag Protein Amino Acid Sequence
Price
$30
Catalog Number
LT0425
Molecular Weight
74 kDa
Description
Recombinant protein containing multiple tags including His, GFP, HA, Flag, GST and Myc
Applications
Western Blot, Immunoprecipitation
Buffer
SDS loading buffer (50 mM Tris pH6.8, 2% SDS, 10% Glycerol, 0.1% bromophenol
blue, 1.5% DTT and 4M Urea)
Concentration
10 ng/uL
Package Size
50 uL
Storage/Handling
Protein storage at -20℃ is recommended. Avoid repeated freeze-thaw cycles
Usage
Loading 5 ul/lane is recommended. For research purposes only

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