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MultiTag Protein: Multiple-Epitope Tagging Loading Control Proteins

A multi-tag loading control protein is used to demonstrate that your western blotting protocol is efficient, and that the antibody recognizes target proteins that might not be present in experimental samples. Loading such positive controls results in the reliable detection of target proteins within samples. It confirms that all steps of the western blot functioned correctly, including gel electrophoresis, transfer to the membrane, membrane blocking, and antibody binding. It also gives you greater confidence that the results obtained from experimental lanes are not artifacts or non-specific binding.

We strongly recommend the use of a positive control protein when setting up new experiments to give you immediate confidence in your western blotting protocol.

When trying to detect low-abundance proteins, it is particularly important to know that your western blot is functioning as expected. If your protein of interest is detected in the control lane, then its absence in other lanes might be related to its low abundance rather than a problem with the blotting protocol.

Highlights:

  • Demonstrate that your protocol is efficient and accurate
  • The ideal positive control protein for your western blot
  • Save time and resources on all your protein research projects
  • Gives you confidence in your western blotting protocol
  • Can be used for immunoprecipitation, affinity purification, western blotting, and dot blots.

Epitope tagging is a technique in which a known epitope is fused to a recombinant protein using genetic engineering. By choosing an epitope for which an antibody is available, it is possible to detect proteins for which no antibody is available. This is particularly useful for the characterization of newly discovered proteins and proteins of low immunogenicity.

By selecting the appropriate epitope and antibody, it is possible to select a combination with properties that are suitable for the desired experimental application, such as western blotting, immunoprecipitation, immunochemistry, or affinity purification.

MultiTag Protein Amino Acid Sequence

MultiTag Protein Amino Acid Sequence
Catalog Number
LT0425
Molecular Weight
72 kDa
Description
Recombinant protein containing mutiple tags including Trx, 6His, GFP, HA, Flag, GST and Myc
Applications
Western Blot, Immunoprecipitation
Buffer
PBS
Concentration
50 ug
Package Size
50ug Lyophilized
Storage/Handling
Store the protein at -20℃ or below. After reconstitution, aliquot and store at -20°C or below.
Usage
Loading 50-100ng is recommended. For research purpose only.
Data Sheet
download pdf data sheet
Epitope Tag Sequences
Trx
MSDKIIHLTD DSFDTDVLKA DGAILVDFWA EWCGPCKMIA PILDEIADEY QGKLTVAKLN IDQNPGTAPK YGIRGIPTLL LFKNGEVAAT KVGALSKGQL KEFLDANLA
6His
HHHHHH
GFP
SKGEELFTG VVPILVELDG DVNGHKFSVS GEGEGDATYG KLTLKFICTT GKLPVPWPTL VTTFSYGVQC FSRYPDHMKQ HDFFKSAMPE GYVQERTIFF KDDGNYKTRA EVKFEGDTLV NRIELKGIDF KEDGNILGHK LEYNYNSHNV YIMADKQKNG IKVNFKIRHN IEDGSVQLAD HYQQNTPIGD GPVLLPDNHY LSTQSALSKD PNEKRDHMVL LEFVTAAGIT HGMDELYK
HA
YPYDVPDYA
FLAG
DYKDDDDK
GST
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSPEFP GRLERPHRD
cMyc
EQKLISEEDL

Related Products:

Anti-DYKDDDDK Monoclonal Antibody: Click here for more product details
download pdf data sheet for more details download pdf data sheet for more details

Anti-His Monoclonal Antibody: Click here to order now

Anti-cMyc Monoclonal Antibody: Click here to order now

Anti-HA Monoclonal Antibody: Click here to order now

Anti-GST Monoclonal Antibody: Click here to order now