| |
| |
Fluorophores list including absorption and emission peak values
|
Fluorophore
|
Absorption
|
Emission
|
|
1,5 IAEDANS
|
336
|
490
|
| 1,8-ANS |
372
|
480
|
| 4-Methylumbelliferone |
385
|
502
|
| 5-carboxy-2,7-dichlorofluorescein |
504
|
529
|
| 5-Carboxyfluorescein
(5-FAM) |
492
|
518
|
| 5-Carboxynapthofluorescein
(pH 10) |
512/598
|
563/668
|
| 5-Carboxytetramethylrhodamine
(5-TAMRA) |
542
|
568
|
| 5-FAM (5-Carboxyfluorescein) |
492
|
518
|
| 5-HAT (Hydroxy
Tryptamine) |
370-415
|
520-540
|
| 5-Hydroxy Tryptamine
(HAT) |
370-415
|
520-540
|
| 5-ROX (carboxy-X-rhodamine) |
578
567
|
604
591
|
| 5-TAMRA (5-Carboxytetramethylrhodamine) |
548
542
|
552
568
|
| 6-Carboxyrhodamine
6G |
518
|
543
|
| 6-CR 6G |
518
|
543
|
| 6-JOE |
520
|
548
|
| 7-Amino-4-methylcoumarin |
351
|
430
|
| 7-Aminoactinomycin
D (7-AAD) |
546
|
647
|
| 7-Hydroxy-4-methylcoumarin |
360
|
449, 455
|
| 9-Amino-6-chloro-2-methoxyacridine |
412, 430
|
471, 474
|
| ABQ |
344
|
445
|
| Acid Fuchsin |
540
|
630
|
| ACMA (9-Amino-6-chloro-2-methoxyacridine) |
412, 430
|
471, 474
|
| Acridine Orange
+ DNA |
502
|
526
|
| Acridine Orange
+ RNA |
460
|
650
|
| Acridine Orange,
both DNA & RNA |
440-480
|
520-650
|
| Acridine Red |
455-600
|
560-680
|
| Acridine Yellow |
470
|
550
|
| Acriflavin |
436
|
520
|
| Acriflavin Feulgen
SITSA |
355-425
|
460
|
| Aequorin (Photoprotein) |
|
466
|
| AFPs - AutoFluorescent
Protein - (Quantum Biotechnologies) see sgGFP, sgBFP |
|
|
| Alexa Fluor 350™ |
346
342
|
442
441
|
| Alexa Fluor 430™ |
431
|
540
|
| Alexa Fluor 488™ |
495, 492
|
519, 520
|
| Alexa Fluor 532™ |
531, 532
|
553, 554
|
| Alexa Fluor 546™ |
556, 557
|
572, 573
|
| Alexa Fluor 568™ |
577, 578
|
603
|
| Alexa Fluor 594™ |
590, 594
|
617, 618
|
| Alexa Fluor 633™ |
632
|
650
|
| Alexa Fluor 647™ |
647
|
666
|
| Alexa Fluor 660™ |
668
|
698
|
| Alexa Fluor 680™ |
679
|
702
|
| Alizarin Complexon |
530-560, 580
|
580, 624-645
|
| Alizarin Red |
530-560
|
580
|
| Allophycocyanin
(APC) |
630, 645
|
655, 660
|
| AMC, AMCA-S |
345
|
445
|
| AMCA (Aminomethylcoumarin) |
345
347
|
425
444
|
| AMCA-X |
353
|
442
|
| Aminoactinomycin
D |
555
|
655
|
| Aminocoumarin |
346
350
|
442
445
|
| Aminomethylcoumarin
(AMCA) |
345
347
|
425
444
|
| Anilin Blue |
|
600
|
| Anthrocyl stearate |
360-381
|
446
|
| APC (Allophycocyanin) |
630, 645
|
655, 660
|
| APC-Cy7 |
625-650
|
755
|
| APTRA-BTC = Ratio
Dye, Zn2+ |
466/380
|
520/530
|
| APTS |
424
|
505
|
| Astrazon Brilliant
Red 4G |
500
|
585
|
| Astrazon Orange
R |
470
|
540
|
| Astrazon Red 6B |
520
|
595
|
| Astrazon Yellow
7 GLL |
450
|
480
|
| Atabrine |
436
|
490
|
| ATTO-TAG™
CBQCA |
465
|
560
|
| ATTO-TAG™
FQ |
486
|
591
|
| Auramine |
460
|
550
|
| Aurophosphine G |
450
|
580
|
| Aurophosphine |
450-490
|
515
|
| BAO 9
(Bisaminophenyloxadiazole) |
365
|
395
|
| BCECF (high pH) |
492, 503
|
520,528
|
| BCECF (low pH) |
482
|
520
|
| Berberine Sulphate |
430
|
550
|
| Beta Lactamase |
409
|
447, 520
|
BFP blue shifted
GFP (Y66H)
Blue Fluorescent Protein |
381, 382, 383
|
445, 447, 448
|
| BFP/GFP FRET |
|
|
| Bimane |
398
|
490
|
| Bisbenzamide |
360
|
461
|
| Bisbenzimide (Hoechst) |
360
|
461
|
| bis-BTC = Ratio
Dye, Zn2+ |
455/405
|
529/505
|
| Blancophor FFG |
390
|
470
|
| Blancophor SV |
370
|
435
|
| BOBO™ -1 |
462
|
481
|
| BOBO™ -3 |
570
|
602
|
| Bodipy 492/515 |
490
|
515
|
| Bodipy 493/503 |
533
|
549
|
| Bodipy 500/510 |
509
|
515
|
| Bodipy 505/515 |
502
|
510
|
| Bodipy 530/550 |
528
|
547
|
| Bodipy 542/563 |
543
|
563
|
| Bodipy 558/568 |
558
|
569
|
| Bodipy 564/570 |
564
|
570
|
| Bodipy 576/589 |
579
|
590
|
| Bodipy 581/591 |
584
|
592
|
| Bodipy 630/650-X |
625
|
642
|
| Bodipy 650/665-X |
647
|
665
|
| Bodipy 665/676 |
605
|
676
|
| Bodipy Fl |
504, 505
|
511, 513
|
| Bodipy FL ATP |
505
|
514
|
| Bodipy Fl-Ceramide |
505
|
511
|
| Bodipy R6G SE |
528
|
547
|
| Bodipy TMR |
542
|
574
|
| Bodipy TMR-X conjugate |
544
|
573
|
| Bodipy TMR-X, SE |
544
|
570
|
| Bodipy TR |
589
|
617
|
| Bodipy TR ATP |
591
|
620
|
| Bodipy TR-X SE |
588
|
616
|
| BO-PRO™ -1 |
462
|
481
|
| BO-PRO™ -3 |
544
|
570
|
| Brilliant Sulphoflavin
FF |
430
|
520
|
| BTC - Ratio Dye
Ca2+ |
464/401
|
533/529
|
| BTC-5N - atio Dye,
Zn2+ |
459/417
|
517/532
|
| Calcein |
494
|
517
|
| Calcein Blue |
373
|
440
|
| Calcium Crimson™ |
588, 589
|
611, 615
|
| Calcium Green |
501, 506
|
531
|
| Calcium Green-1
Ca2+ Dye |
506
|
531
|
| Calcium Green-2
Ca2+ |
506/503
|
536
|
| Calcium Green-5N
Ca2+ |
506
|
532
|
| Calcium Green-C18
Ca2+ |
509
|
530
|
| Calcium Orange |
549
|
575
576
|
| Calcofluor White |
385, 395, 405
|
437, 440, 445
|
| Carboxy-X-rhodamine
(5-ROX) |
576
|
601
|
| Cascade Blue™ |
377
398
399
|
420
423
|
| Cascade Yellow |
399
400
|
550
552
|
| Catecholamine |
410
|
470
|
| CCF2 (GeneBlazer) |
|
|
| CFDA |
494
|
520
|
| CFP - Cyan Fluorescent
Protein |
430, 433, 436, (453)
|
474, 475, 476, (501)
|
| CFP/YFP FRET |
|
|
| Chlorophyll |
480
|
650
|
| Chromomycin A |
436-460
|
470
|
| Chromomycin A |
445
|
575
|
| CL-NERF (Ratio
Dye, pH) |
504/514
|
540
|
| CMFDA |
494
|
520
|
| Coelenterazine
Ca2+ Dye, bioluminescence |
(429)
|
465
|
| Coelenterazine
cp (Ca2+ Dye,) |
(430)
|
442
|
| Coelenterazine
f |
(437)
|
473
|
| Coelenterazine
fcp |
|
452
|
| Coelenterazine
h |
(437)
|
464
|
| Coelenterazine
hcp |
(433)
|
444
|
| Coelenterazine
ip |
|
441
|
| Coelenterazine
n |
(431)
|
467
|
| Coelenterazine
O |
460
|
575
|
| Coumarin Phalloidin |
387
|
470
|
| C-phycocyanine |
|
|
| CPM Methylcoumarin |
384
|
469
|
| CTC |
400-450
|
602
|
| CTC Formazan |
|
|
| Cy2™ |
489
|
506
|
| Cy3.1 8 |
554
|
568
|
| Cy3.5™ |
581
|
598
|
| Cy3™ |
514
552
554
|
566
570
|
| Cy5.1 8 |
649
|
666
|
| Cy5.5™ |
675
|
695
|
| Cy5™ |
649
|
666
670
|
| Cy7™ |
710, 743
|
767, 805
|
| Cyan GFP |
433 (453)
|
475 (501)
|
| cyclic AMP Fluorosensor
(FiCRhR) |
500
|
517
|
| CyQuant Cell Proliferation
Assay |
480
|
520
|
| Dabcyl |
453
|
|
| Dansyl |
340
|
578
|
| Dansyl Amine |
337
|
517
|
| Dansyl Cadaverine |
335
|
518
|
| Dansyl Chloride |
372
|
518
|
| Dansyl DHPE |
336
|
517
|
| Dansyl fluoride |
356
|
none
|
| DAPI |
359
|
461
|
| Dapoxyl |
403
|
580
|
| Dapoxyl 2 |
374
|
574
|
| Dapoxyl 3 |
373
|
574
|
| DCFDA |
504
|
529
|
| DCFH (Dichlorodihydrofluorescein
Diacetate) |
505
|
535
|
| DDAO |
463
|
607
|
| DHR (Dihydorhodamine
123) |
505
|
534
|
| Di-4-ANEPPS |
496
|
705
|
| Di-8-ANEPPS (non-ratio) |
488
498
|
605
713
|
| DiA (4-Di-16-ASP) |
456
|
591
|
| Dichlorodihydrofluorescein
Diacetate (DCFH) |
505
|
535
|
| DiD - Lipophilic
Tracer |
644
|
665
|
| DiD (DiIC18(5)) |
644
|
665
|
| DIDS |
341
|
415
|
| Dihydorhodamine
123 (DHR) |
505
|
535
|
| DiI (DiIC18(3)) |
549, 551
|
565
|
| Dinitrophenol |
349
|
|
| DiO (DiOC18(3)) |
484, 487
|
501, 502
|
| DiR |
748
|
780
|
| DiR (DiIC18(7)) |
750
|
779
|
| DM-NERF (high pH) |
497/510
|
540
|
| DNP |
349
|
|
| Dopamine |
340
|
490-520
|
| DsRed |
558
|
583
|
| DTAF |
494
|
520
|
| DY-630-NHS |
621
|
660
|
| DY-635-NHS |
634
|
664
|
| EBFP |
383
|
447
|
| ECFP |
436
|
474
|
| EGFP |
488, 498
|
507, 516
|
| ELF 97 |
345
|
530
|
| Eosin |
524
|
545
|
| Erythrosin |
529, 532
|
554, 555
|
| Erythrosin ITC |
529
|
555
|
| Ethidium Bromide |
510, 523
|
595, 605
|
| Ethidium homodimer
-1 (EthD-1) |
528
|
617
|
| Euchrysin |
430
|
540
|
| EukoLight |
|
|
| Europium (III)
chloride |
|
|
| EYFP |
513, 520
|
527, 532
|
| Fast Blue |
360
|
440
|
| FDA |
494
|
520
|
| Feulgen (Pararosaniline) |
570
|
625
|
| FIF (Formaldehyd
Induced Fluorescence) |
405
|
433
|
| FITC |
490, 494
|
520, 525
|
| FITC Antibody |
493
|
517
|
| Flazo Orange |
375-530
|
612
|
| Fluo-3 |
480 – 506, 506
|
520, 527
|
| Fluo-4 |
494
|
516
|
| Fluorescein (FITC) |
490, 494
|
520, 525
|
| Fluorescein Diacetate |
494
|
520
|
| Fluoro-Emerald |
495
|
524
|
| Fluoro-Gold (Hydroxystilbamidine) |
361
|
536
|
| Fluor-Ruby |
555
|
582
|
| FluorX |
494
|
520
|
| FM 1-43™ |
479
|
598
|
| FM 4-46 |
515
|
640
|
| Fura Red™
(high pH) |
572
|
657
|
| Fura Red™/Fluo-3 |
|
|
| Fura-2, high calcium |
335
|
505
|
| Fura-2, low calcium |
363
|
512
|
| Fura-2/BCECF |
|
|
| Genacryl Brilliant
Red B |
520
|
590
|
| Genacryl Brilliant
Yellow 10GF |
430
|
485
|
| Genacryl Pink 3G |
470
|
583
|
| Genacryl Yellow
5GF |
430
|
475
|
| GeneBlazer (CCF2) |
|
|
| GFP (S65T) |
498
|
516
|
| GFP red shifted
(rsGFP) |
498
|
516
|
| GFP wild type,
non-UV excitation (wtGFP) |
475
|
509
|
| GFP wild type,
UV excitation (wtGFP) |
395
|
509
|
| GFPuv |
385
|
508
|
| Gloxalic Acid |
405
|
460
|
| Granular Blue |
355
|
425
|
| Haematoporphyrin |
530-560
|
580
|
|
