LifeTein Peptide Modifications: Multiple Antigenetic Peptides

Multiple Antigenic Peptides (MAP) is artificial branched peptides. Lysine residues are used as scaffolding core to form up to 8 branches with the same or different peptide sequences. MAP have been used for immunological studies for antibody production. Some peptides render low immunological response. But with concentrated peptides in branched forms, the new structure can dramatically increase the immunological response when injected into the animals.

Multiple antigenic peptides can be synthesized by standard SPPS. Boc-Lys(Boc)-OH is anchored to resin . After treatment with TFA, deprotection, coupling, and deprotection cycles are performed sequentially. The peptides for the immunological studies are then synthesized on each of the eight branches.

The branched proteins are unknown in nature. And the synthesis of MAPs is not straightforward. The stringent space between 8 branches could lead to aggregation of the peptides on the resin and produce low coupling yields and peptide deletions.

PeptideSyn technology is used by LifeTein to overcome the problem by a chemical ligation strategy. This strategy will give desired peptide dendrimer at good yield. The branched structures could form big molecular weight of proteins for the immunogen purpose.

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