LifeTein Peptide Modifications: Multiple Antigenetic Peptides

Multiple Antigenic Peptides (MAP) are artificially branched peptides. Lysine residues are used as a scaffolding core to form up to 8 branches with the same or different peptide sequences. MAP has been used for immunological studies for antibody production. Some peptides render a lower immunological response. However with concentrated peptides in branched forms, the new structures can dramatically increase the immunological response when injected into animals.

Multiple antigenic peptides can be synthesized by standard SPPS. During the process, Boc-Lys(Boc)-OH is anchored to a resin . After treatment with TFA, deprotection, coupling, and deprotection cycles are performed sequentially. The peptides for the immunological studies are then synthesized on each of the eight branches.

The branched proteins are unknown in nature. The synthesis of MAP is not generally straightforward. The stringent space between the 8 branches could lead to aggregation of the peptides on the resin and produce low coupling yields and peptide deletions.

PeptideSyn technology is used by LifeTein to overcome this problem by a chemical ligation strategy. This strategy will give the desired peptide dendrimer at higher yield. The branched structures could then form a greater molecular weight of protein for the immunogen purpose.

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