Anti cMyc Monoclonal Antibody

Catalog Number:	LT0421
Lot Number:	411005100204
Isotype:	Mouse IgG1
Clonality:	Myc.A7
Quantity:	50 μg, lyophilized with PBS, pH7.4
Positive Control:	Purchase matching Western Blot positive control protein
Immunogen:	Synthetic c-Myc tag EQKLISEEDL peptide conjugated to KLH
Specificity:	Recognizes the N-terminal or C-terminal Myc-tagged fusion proteins
Purification:	Protein A affinity chromatography from mouse ascites fluid
Applications:	Dot, ELISA, IP, IS, WB
Sensitivity:	WB (with ECL): 10-50ng of Myc-tagged fusion proteins
Working Condition:	WB (with ECL): 1:1000-3000 dilution (incubate for 1 hour at room temperature) IS: 1:500-2000 dilution For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test
Storage:	Store at -20°C for up to 2 years. Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 50ug/50ul. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for 1-2 weeks
Images:	Fig. 1:1000 (1µg/mL) Ab dilution probed against HEK293 cells transfected with Myc-tagged protein vector; untransfected (1) and transfected (2)

Other related products:

Cat. No	Product Name	Applications	Price	Order
5355	Lys(Azide) cMyc tag peptide for click chemistry c-Myc tag EQKLISEEDL	Recognizes the N-terminal or C-terminal Myc-tagged fusion proteins, Anti cMyc Monoclonal Antibody	$150
LT8282	HA tag Peptide C-YPYDVPDYA	YPYDVPDYA motif, Anti HA Monoclonal Antibody	$250
LT12006	HHHHHH-Cysteine	6His-Cysteine, Anti His Monoclonal Antibody	$99
5989	{Cy5}-Cys-HHHHHHHHHLPETGG	Fluorescent Labeling	$480
LT12022	FLAG peptide, DYKDDDDK	DYKDDDDK-Cysteine; Anti DYKDDDDK Monoclonal Antibody	$88
LT8281	GLNDIFEAQKIEWHE	AviTag	$250