The original DYKDDDDK peptide (the same epitope recognized by Sigma's Anti-FLAG® **antibodies) was an 11-amino-acid leader peptide of the gene-10 product from bacteriophage T7 fused at the amino-terminus of GAL4 (yeast transcription factor). The binding reaction of this tag to the anti-DYKDDDDK antibody is calcium dependent, so proteins can frequently be eluted from an affinity matrix by buffers containing EDTA. It also allows for the tag to be placed at the amino-terminus, at the carboxy-terminus, or in association with other tags.
LifeTein's anti-DYKDDDDK monoclonal antibody was developed based on the most widely used hydrophilic octapeptide: DYKDDDDK. LifeTein's anti-DYKDDDDK 1E6 renders higher affinity and higher binding capacity than Sigma's antibody. Please see the following table for technical details.
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Catalog Number:
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LT0420 |
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Lot Number:
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411005100203 |
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Isotype:
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Mouse IgG2b |
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Clone No:
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FG4R |
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Source:
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Mouse ascites |
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Quantity:
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50 ug |
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Description:
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Monoclonal antibodies are produced by immunizing mouse with synthetic peptide (DYKDDDDK), coupled to KLH. |
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Applications:
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Western Blot, Immunoprecipitation |
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Buffer:
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PBS with 50% glycerol; protein concentration: 0.5 mg/ml |
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Recommended Dilutions:
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Western Blot: 0.5 ug/ml |
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Storage:
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Storage at -20 C is recommended. Avoid repeated freeze-thaw cycle. |
*FLAG is a registered trademark of Sigma-Aldrich Co. LLC
**ANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC
