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Anti-Flag Monoclonal Antibody
The flag peptide that was first used was a 11-amino-acid leader peptide of the gene-10 product from bacteriophage T7 fused at the amino-terminus of GAL4 (yeast transcription factor).The binding reaction of this tag to the anti-flag antibody is Calcium dependent, so proteins can frequently be eluted from an affinity matrix by EDTA containing buffer and it allows for the tag to be placed at either the amino-terminus, carboxy-terminus, or in association with other tags.
LifeTein's anti-flag monoclonal antibody was developed based on the most widely used hydrophilic octapeptide:DYKDDDDK. LifeTein's anti-Flag 1E6 renders higher affinity or higher binding capacity than Sigma's antibody. Please see the following table for technical details.
Comparison of Anti-Flag 1E6 affinity gel with commercially available M2 affinity gel 
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| $50 | |
| LT0420 | |
| 411005100203 | | Mouse IgG2b | | 1E6 | | Mouse Ascites | | 50 μg | | Monoclonal antibodies were produced by immunizing mouse with synthetic peptide (DYKDDDDK), coupled to KLH. | | Western Blot, Immunoprecipitation | | 1×PBS with 50% glycerol; Protein concentration: 0.5 mg/ml | | Western Blot: 0.5 μg/ml | | Antibody is recommended to be stored at -20 C. Avoid repeated freeze-thaw cycle. |
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