Selected Publications
LifeTein® provides custom chemical synthesis, custom peptide synthesis, and custom antibody products and services to support academic institutions, government research facilities, and pharmaceutical and biotech companies locally and worldwide to accelerate the process of biological research. Our practical chemistry often involves molecular interactions in multiple phases beyond gaseous collisions. LifeTein's research and development efforts involve continuous attempts to improve and find alternative peptide synthesis routes and provide guidance on protein structure studies. With the help of LifeTein scientists, our customers have made many breakthroughs and developed many innovations relevant to fields of research including proteomics and cell biology.
Success Stories: References using synthetic peptides and antibodies from LifeTein.
Structure–activity studies of Drosophila adipokinetic hormone (AKH) by a cellular expression system of dipteran AKH receptors, 2012, J Caers, L Peeters, et al., General and Comparative Endocrinology, http://dx.doi.org/10.1016/j.ygcen.2012.04.025
Gade's lab, using synthetic peptide hermone from LifeTein, found that the amino acids 1–5 of endogenous adipokinetic hormone of Drosophila melanogaster (pGlu-Leu-Thr-Phe-Ser-Pro-Asp-Trp-NH2) are crucial for receptor activation and probably form a β-strand. Structure–activity studies for the adipokinetic hormone receptor of insects were for the first time performed in a cellular expression system.
... The peptides for the SAR analyses were synthesized by LifeTein LLC (South Plainfield, USA).
To dissolve the peptides, 80% acetonitrile was used. Purification of the peptides was performed
with reversed-phase high performance liquid chromatography (HPLC)...
Non-muscle myosin IIA transports a Golgi glycosyltransferase to the endoplasmic reticulum by binding to its cytoplasmic tail, 2012,Armen Petrosyan, et al, The International Journal of Biochemistry & Cell Biology, http://dx.doi.org/10.1016/j.biocel.2012.04.004
Armen Petrosyan from Pi-Wan Cheng's lab, using biotin labeled synthetic peptide from LifeTein, found that non-muscle myosin IIA is involved in the Golgi remodeling. NMIIA and not coat protein complex-I is responsible for transporting the Golgi glycosyltransferase to the ER for proteasomal degradation.Inhibition of non-muscle myosin IIA prevents brefeldin A-induced Golgi collapse.
... A 21 aa peptide, which includes the cytoplasmic tail (MVQWKRLCQLHYL) and a portion of
the transmembrane domain (WALGCYM) located at the N-terminus of hC2GnT-M, was tagged
with a biotin at the C-terminus ((LifeTein) LLC, South Plainfield, NJ)...
Structures of KIX domain of CBP in complex with two FOXO3a transactivation domains reveal promiscuity and plasticity in coactivator recruitment, Feng Wang, Christopher B. Marshall,et al, Published online before print April 2, 2012, doi: 10.1073/pnas.1119073109, PNAS April 2, 2012
Feng Wang from Ikura's lab, using phosphorylated synthetic peptide from LifeTein, identified the increased affinity for CBP/p300 and the phosphomimetic mutation enhanced transactivation of luciferase.These findings underscore the significance of promiscuous multivalent interactions and posttranslational modification in the recruitment of transcriptional coactivators, which may allow transcription factors to adapt to various gene-specific genomic and chromatin structures and respond to cell signals.
... CR3Trp: CR3 with the insertion of a Trp residue at the N terminus (W-L620-A635) in pGEX4T-1 vector; pCR3: Synthetic CR3Trp peptide with S626 phosphorylated (LifeTein) ... supplement data
Binding interface of cardiac potassium channel proteins identified by hydrogen deuterium exchange of synthetic peptides; J Chen, R Angeletti, TV McDonald… - Analytical and Bioanalytical Chemistry, 2012
Synthetic human potassium channel proteins Ether-a-go-go-related gene (HERG) peptides, synthesized by LifeTein, were used to study the protein structure and peptide binding activities. The residues determined to be involved in binding were consistent with a cysteine cross-linking study and confirmed by double mutant cycle analysis.
... All three peptides including the peptide KCNE1 67–86 (RSKKLEHSNDPFNVYIESDA) and KCNQ1 358–374 (KQRQKHFNRQIPAAASL), as well as HERG N-terminal peptide (MPVRRGHVAPQNTFLDTIIRKFEGQS) were synthesized by LifeTein LLC (South Plainfield, NJ, ...
Connexin-43 hemichannels contribute to cytoplasmic Ca2+ oscillations by providing a bimodal Ca2+-dependent Ca2+-entry pathway, Marijke De Bock, Nan Wang, Melissa Bol, Elke Decrock, Raf Ponsaerts, Geert Bultynck, Genevieve Dupont and Luc Leybaert1, The Journal of Biological Chemistry, 2012, http://www.jbc.org/cgi/doi/10.1074/jbc.M111.299610
Researchers from Ghent University found that the Connexin hemichannels may contribute to Ca2+ oscillations
in connexin expressing cells. Synthetic peptides synthesized by LifeTein were used for the hemichannel assays.
......32Gap27 (SRPTEKTVFT, amino acids 182 to 191 in the second extracellular loop of Cx32), 43Gap26 (VCYDKSFPISHVR, amino acids 64 to 76 in the first extracellular loop of Cx43), CT9 (RPRPDDLEI, last 9 amino acids of the Cterminal tail of Cx43), CT9DI (RPRPDDLE), the reversed CT9 peptide (CT9Rev, IELDDPRPR),
YGRKKRRQRRR-CT9 (Tat-CT9) and Tat-CT9Rev were synthesized by LifeTein (New Jersey, USA) at > 80 % purity...
Human apolipoprotein E peptides inhibit hepatitis C virus entry by blocking virus binding, Hepatology, 2012, Shufeng Liu1, Kevin D. McCormick1, Wentao Zhao, Ting Zhao, Daping Fan, Tianyi Wang,DOI: 10.1002/hep.25665
Wang lab found that the apoE peptides potently inhibit HCV infection and suggest a direct role of apoE in mediating HCV entry. Peptides were synthesized by LifeTein. Their findings also highlight the potential of developing apoE mimetic peptides as novel HCV entry inhibitors by targeting HCV-host interactions.
......Human apolipoprotein E peptides inhibit hepatitis C virus entry by blocking virus ... apolipoprotein E, viral hepatitis, viral entry, peptide inhibitor, virus ...... peptides (hEP1-12, Table 1) were chemically synthesized by LifeTein LLC...
Structural Basis of Response Regulator Inhibition by a Bacterial Anti-Activator Protein, Melinda D. Baker and Matthew B. Neiditch, PLoS Biol. 2011 December; 9(12): e1001226, doi: 10.1371/journal.pbio.1001226
Neiditch lab used synthetic peptide PhrF from LifeTein and found that the PhrF binding to RapF allosterically triggers its dissociation from ComA. The data revealed the first atomic level insight into the inhibition of response regulator DNA binding by an anti-activator.
...Gel filtration runs were performed with RapF, His-ComA, and PhrF (NH2-QRGMI-COOH) (LifeTein) at 20 µM, 40 µM, and 200 µM, respectively...
Functional impact of silencing the Helicoverpa armigera sex‐peptide receptor on female reproductive behaviour, O Hanin, A Azrielli, SW Applebaum, A. Rafaeli, Insect Molecular Biology, Dec 16, 2011, DOI: 10.1111/j.1365-2583.2011.01122.x
Rafaeli lab used sex peptide, synthesized by LifeTein, to show that virgin females injected with Drosophila melanogaster SP (DrmSP), in addition to inhibition of pheromone production, also exhibited a suppression of calling behaviour and a significant reduction in the gene expression levels of the PBAN-receptor.
...l phage DE3 ea59 dsRNA (control) or DEPC-treated water (control) were injected during the third hour of the second scotophase with 10 µl DrmSP (10 pmol/ µl; LifeTein, South Plainfield ...
ACS Nano, Dec 13, 2011, DOI: 10.1021/nn204361s, Multiplex Sensing of Protease and Kinase Enzyme Activity via Orthogonal Coupling of Quantum Dot-Peptide Conjugates, Stuart B. Lowe, et al.
Stuart B. Lowe developed a detection system capable of simultaneous evaluation of the activity of two critical enzyme classes, proteases and kinases. Peptide substrates were synthesized by LifeTein.
... al.36 Peptides. uPA substrate peptide (biotin-SGRSANC-CONH2) & Her2 control peptide (HHHHHHGGGDNE(pY)FYV-CONH2) were purchased from LifeTein. Her2 substrate peptide (HHHHHHGGGDNEYFYV-CONH2)...
Front Microbiol. 2011; 2: 128. 10.3389/fmicb.2011.00128, Susceptibility of Pseudomonas aeruginosa biofilm to alpha-helical peptides: D-enantiomer of LL-37, Scott N. Dean, et al.
Scott N. Dean demonstrated that a protease-resistant LL-37 peptide mimetic, the peptide enantiomer D-LL-37, could be a critical advancement in the development of new treatments for P. aeruginosa infection. This enantiomer D-LL-37 peptide was synthesized by LifeTein.
...The anti-microbial activity of the NA-CATH and NA- CATH: … and D-LL-37 ((LifeTein), South Plainfield, NJ,USA)against P.aeruginosa (ATCC19429)...
Nature Immunology, (2011), doi:10.1038/ni.2107, The carboxypeptidase ACE shapes the MHC class I peptide repertoire, Xiao Z Shen, et al.
Xiao Z Shen from Bernstein’s lab found that the carboxypeptidase angiotensin-converting enzyme shapes the MHC class I peptide repertoire. He proposed a revised model of peptide processing for MHC class I by introducing carboxypeptidase activity into the process.
...The peptides Smcy (KCSRNRQYL) and Uty (WMHHNMDLI) were from …. All other peptides were synthesized and purified by high-performance liquid chromatography by (LifeTein)...
PNAS, Aug 2011; 108: 13275 - 13280. Binding of Plasmodium merozoite proteins RON2 and AMA1 triggers commitment to invasion. Prakash Srinivasan, et al.
Prakash Srinivasan identified that RON2, a parasite protein, could be a potential vaccine candidate. RON2 peptides were synthesized by LifeTein.
...Peptides derived from PfRON2 were custom synthesized by LifeTein LLC. Their sequences are listed in Fig.2B. A biotin tag and a 6-carbon aminohexanoic acid (Ahx) linker were added to the N terminus. The thiol group in the cysteine side chains was blocked (by alkylation) according to proprietary methods (LifeTein LLC)...
J. Bacteriol. doi:10.1128/JB.05860-11, 9 September 2011, An atypical Phr peptide regulates the developmental switch protein RapH, Nicolas Mirouze, et al.
UMDNJ-New Jersey Medical School scientists found an atypical Phr peptide regulates the developmental switch protein RapH. PhrH is the prototype of a newly identified class of Phr signaling molecules consisting of six amino acids. The synthetic peptides were from LifeTein.
...The synthesized peptides, TDRNTTY, TDRNTT, DRNTTY and DRNTT, 193 were obtained from LifeTein... 194 Concentrations were adjusted by resuspending the lyophilized peptides (weight provided by 195 LifeTein) in the appropriate buffer volume...
Plant Physiology, Sep 2011; 157: 476 - 484. Mechanisms of Molecular Mimicry of Plant CLE Peptide Ligands by the Parasitic Nematode Globodera rostochiensis. Yongfeng Guo, et al.
Yongfeng Guo from Clark’s lab identified two host factors involved in the function of a receptor ligand mimic. The fluorogenic substrate K was synthesized by LifeTein.
...The fluorogenic substrate K(Dabcyl)-TLEEQKRVTPGGPDPLHNRE-EDANS was synthesized by LifeTein with a purity of greater than 90%. A stock solution of 1 mm was made in dimethyl sulfoxide (DMSO), and the final concentration...
Nucleic Acids Res., Aug 2011; 39: 6305 - 6314. Mechanism of RecO recruitment to DNA by single-stranded DNA binding protein. Mikhail Ryzhikov, et al.
Korolev’s lab found that RecO does not bind SSB-Ct and weakly interacts with the peptide in the presence of RecR, suggesting the diverse mechanisms of DNA repair pathways mediated by RecO in different organisms.
...Peptides were initially purchased from … and later from LifeTein LLC (South Plainfield, NJ, USA). SSB-Ct peptide had an additional N-terminal Trp residue for quantification (WYMDFDDDIPF-177...
Insect Biochemistry and Molecular Biology, Volume 41, Issue 8, August 2011, Pages 537-544, Identification and differential expression of a sex-peptide receptor in Helicoverpa armiger. Orly Hanin et al.
Rafaeli’s lab identified a putative receptor for sex-peptide (SP-R) in H. armigera on the basis of sequence homologies deposited in the GenBank. A series of peptides were synthesized by LifeTein.
...were decapitated 1 hour after mating to exclude endogenous PBAN release and injected with PBAN (5 pmol/female) in the presence or absence of various doses of synthetic DrmSP (5, 10, 50 and 100 pmol/female) (LifeTein, South Plainfield ...
BMC Microbiology 2011, 11:114doi:10.1186/1471-2180-11-114, Natural and synthetic cathelicidin peptides with anti-microbial and anti-biofilm activity against Staphylococcus aureus, Scott N Dean et al.
Monique L van Hoek’s lab found that the highly-effective anti-microbial peptide NA-CATH:ATRA1-ATRA1 not only displayed improved anti-biofilm activity relative to parent peptide, but it also exhibited enhanced anti-microbial activity. Peptide D-LL-37 synthesized by LifeTein may possesses wound-healing properties towards the host.
...ATRA-1, ATRA-1A, ATRA-2 peptides (86.1 and 89.7, 97.2, 94.5, and 88.2%, respectively), LL-37 (95% purity) and D-LL-37 (92.0% purity) (Lifetein, South Plainfield, NJ) were synthesized...
Mechanisms of Development, doi:10.1016/j.mod.2011.09.002, Drosophila spoonbill encodes a dual-specificity A-kinase anchor protein essential for oogenesis, Meytal Hadad, et. Al.
Israel scientists found found spoonbill to be allelic to CG3249 (also known as yu), which encodes the only known dual-specificity A-kinase anchor protein in Drosophila. The polyclonal antibodies again GST-Spoon/Yu fusion protein was produced by LifeTein.
...The 50 kDa GST- Spoon/Yu fusion protein produced upon induction was used for custom made polyclonal antibodies in rabbits (LifeTein LLC, USA). 4.4. RNA in situ hybridization and immunohistochemistry In situ was as in Tautz and Pfeifle (1989)...
Cell Death and Differentiation advance online publication 5 August 2011; doi: 10.1038/cdd.2011.97, Selective regulation of IP3-receptor-mediated Ca2+signaling and apoptosis by the BH4 domain of Bcl-2versus Bcl-Xl, G Monaco et al.
Using biotinylated peptide custom synthesized from LifeTein, Bultynck’s lab from Ghent University revealed a difference in the cellular activity of the BH4 domain of Bcl-2 and Bcl-Xl toward IP3R regulation because of a single residue difference between both domains.
... Biotinylated peptides were obtained from LifeTein (South Pleinfield, NJ, USA). Table 1 ...
MiniGenes to Peptides - Exploring and Exploiting Ion Channel Function; Decoded, Vol. 1, No.1, April 2011.
IDT and Ghent University scientists used peptides from LifeTein to study ion channel function. They developed channel peptide fragments to identify their critical determinants.
...the laboratory uses chemically synthesized peptides from commercial sources (LifeTein)...
MiniGenes to Peptides - Exploring and Exploiting Ion Channel Function; Decoded, Vol. 1, No.1, April 2011.
IDT and Ghent University scientists used peptides from LifeTein to study ion channel function. They developed channel peptide fragments to identify their critical determinants.
...the laboratory uses chemically synthesized peptides from commercial sources (LifeTein)...
Biochemical and Biophysical Research Communications, Volume 407, Issue 3, 15 April 2011, Pages 512-516, Upregulation of SIRT1 deacetylase in phenylephrine-treated cardiomyoblasts, Catherine L. Passariello et al.,
University of Florence scientists used synthetic peptides from LifeTein to study the sirtuin SIRT1, which plays a role in biological processes such as longevity and stress response.
...tetrapeptide RHKKAc AMC [23] purchased from LifeTein, South Plainfield, NJ. This peptide is based on the amino acid sequence of human p53 close to Lys382...
Cellular Immunology, Volume 267, Issue 2, 2011, Pages 77-87, Taenia crassiceps infection abrogates experimental autoimmune encephalomyelitis, José L. Reyes et al.,
Terrazas lab used myelin–oligodendrocyte glycoprotein (MOG) synthetic peptide 35–55 from LifeTein and showed Taenia crassiceps infection abrogates experimental autoimmune encephalomyelitis.
... as well as uninfected mice, were immunized subcutaneously with 300 μg myelin oligodendrocyte glycoprotein peptide 35–55 (MOG35–55) (Lifetein, ...
Biotechnol Lett, DOI 10.1007/s 10529-011-0519-6, Selection of silk-binding peptides by phage display, Yoko Nomura et al.,
Dr. Yokobayashi group at UC Davis scientists used synthetic peptide and identified a consensus sequence QSWS which is important for silk-binding. The silk-binding peptides will be useful for functionalization of silk for biomaterial applications.
... Peptides were chemically synthesized with C-terminal biotin by LifeTein. Each 12-mer peptide was synthesized...
Nucl. Acids Res. (2011) doi: 10.1093/nar/gkr199, Mechanism of RecO recruitment to DNA by single-stranded DNA binding protein, Mikhail Ryzhikov, et al.,
Mechanism of RecO recruitment to DNA by single-stranded DNA binding protein was revealed by Korolev group. The synthetic SSB C-terminus (SSB-Ct) peptides were synthesized by LifeTein. The single-stranded (ss) DNA binding protein, SSB, plays an inhibitory role by protecting ssDNA from annealing and recombinase binding.
... Peptides were initially purchased … from LifeTein LLC (South Plainfield, NJ, USA). ...
Electron capture induced dissociation of dipeptide dications: where does the charge go? Camilla Skinnerup Jensen, Jean Ann Wyer and Steen Brøndsted Nielsen, Phys. Chem. Chem. Phys., 2010, 12, 12961-12963
LifeTein helped scientists answer this question: electron capture induced dissociation of dipeptide dications: where does the charge go? LifeTein synthesized the dipeptides.
... QK was purchased from ... from LifeTein LLC. ...
LifeTein helped characterize the immune response to HIV-1 using host derived epitope R7V. LifeTein synthesized R7V peptide and produced polyclonal R7V antibodies.
Christiane Bremnas, Debra Meyer, University of Pretoria, South Africa
...LifeTein's PeptideSyn platform was used for the synthesis of the R7V peptide... The peptide and polyclonal antibodies were commercially manufactured by LifeTein LLC...
The Structure of Calreticulin C-terminal Domain Is Modulated by Physiological Variations of Calcium Concentration.
Ana Maria Villamil Giraldo,Maximo Lopez Medus, Mariano Gonzalez Lebrero, Rodrigo S. Pagano, Carlos A. Labriola, Lucas Landolfo, Jose M. Delfino, Armando J. Parodi and Julio J. Caramelo.
December 15, 2009, doi: 10.1074/jbc.M109.034512 February 12, 2010 The Journal of Biological Chemistry, 285, 4544-4553.
Selection for Non-Amyloidogenic Mutants of Islet Amyloid Polypeptide (IAPP) Identifies an Extended Region for Amyloidogenicity.
Ayano Fox , Thibaut Snollaerts, Camille Errecart Casanova, Anastasia Calciano, Luiza A Nogaj and David A. Moffet
Biochemistry, Just Accepted Manuscript DOI: 10.1021/bi100337p Publication Date (Web): August 10, 2010
Aug 10, 2010 ... except for A13E IAPP (synthesized by LifeTein LLC, South Plainfield, NJ). ...
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