Custom Polyclonal Antibody Services

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A: (Cat. No. LA001) Standard polyclonal antibody services using peptide antigens

1. Peptide antigen design (free), Peptide synthesis: >75% purity, 10 mg, up to 15 amino acids
2. Conjugation of peptide to KLH (2-3 mg)
3. Standard 12-15 week antibody production schedule including 4 immunizations with 2 rabbits
4. Affinity purification of selected batch of serum. Deliverables: 2-3 mg peptides, 1-2 ml pre-immune sera, 2-5 mg purified antibodies, ELISA results (>1:32,000)

B: (Cat. No. LA002) Polyclonal antibody services from gene to antibodies

1. Gene amplification, vector construction, protein expression in E. coli.
2. Immunization, antigen affinity purification
3. Standard 14-17 week antibody production schedule
4. Deliverables: construct vector, 1 mg protein, 1-2 ml pre-immune sera, 2-5 mg purified antibodies, ELISA results (>1:32,000)

C: (Cat. No. LA003) Standard polyclonal antibody services using phospho-peptide antigens

1. Peptide antigen desing (free) and peptide synthesis
   • Phosphopeptide: >95% purity, 10 mg, up to 15 amino acids
   • Non-phosphopeptide: >75% purity, 10 mg, up to 15 amino acids
2. Conjugation of phosphopeptide to KLH or BSA (2-3 mg); conjugation of the non-phosphorylated peptide to BSA
3. Standard 13-16 week antibody production schedule including 4 immunizations with 2 rabbits
4. Affinity purification of selected batch of serum; depletion of non-phosphospecific antibodies; purification of phosphospecific antibodies
5. Deliverables: 1 pre-immune serum collection, 2-3 mg peptides (unmodified and phospho), 1-2ml pre-immune sera, 2-5 mg phospho-specific antibodies, ELISA results (>1:32,000)

D: (Cat. No. LA004) Western-guaranteed polyclonal antibodies

1. Peptide design (free) and peptide synthesis
2. Conjugation of peptide to carrier
3. Standard 15-18 week antibody production schedule
4. Affinity purification of selected batch of serum
5. Deliverables: 2-3 mg peptides, 1-2 ml pre-immune sera, 2-5 mg purified antibodies, ELISA results >1:32,000, Western blot test results of 1:200 for the antibody generated from at least one peptide

E: Additional services:

• 4-Week extension: We provide 1 additional immunization and 2 additional bleed collections for approximately 80-100ml of polyclonal antisera
• Affinity purification: Affinity purification of 25 ml of sera, including the affinity column for running additional purifications
• ELISA: On any bleed or purified antibody, upon requests
• Western blot: Assay of antisera or purified antibodies against the antigens, upon request
• Immunochemistry: Assay of purified antibodies against pathological slices, upon request

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