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Anti-ERK1 (E32) Monoclonal Antibody

$260.00

Calatog Number:
LT9997
Lot Number:
411005100213
Isotype:
Mouse IgG1
Clonality:
E32
Quantity:
50 μg
Immunogen:
Recombinant full length ERK1 protein
Specificity:
Recognizes only the ERK1 protein (e.g.: from human and mouse cells)
Cross Reactivity:
WB: ERK1 from human and mouse cells. IP: ERK1 from human cells. ERK1 from other species may also be detectable
Purification:
Protein A affinity chromatography from mouse ascites fluid
Applications:
IP, WB
Working Condition:
WB (with ECL): 1:1000-10,000 dilution (incubate for one hour at room temperature)
For best results with other assays (e.g.: IP, etc), please determine optimal working dilution by titration test
Storage:
Store at -20C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4C for 1-2 weeks
Images:
anti-ERK1 (E32) mAb dilution used in WB of HEK293 and HELA cell lysateanti-ERK1 (E32) mAb dilution used in WB of HEK293 and HELA cell lysate
Fig. LEFT: 1:1,000 (1g/mL) Ab dilution used in WB of HEK293 cell lysate; 5g (1), 10g (2), and 30g (3) of cell lysate used; RIGHT: IP of anti-ERK1 (1L) using HeLa cell lysate; 10g (1), 25g (2), and 50g (3) of cell lysate used

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1-9
$260.00
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$234.00
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$208.00
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$182.00

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