Anti-GFP Monoclonal Antibody

Catalog Number:	LT9994
Lot Number:	411005100210
Isotype:	Mouse IgG1
Clonality:	GF28R
Quantity:	50 μg, Lyophilized with PBS, pH7.4
Positive Control:	Purchase matching Western Blot positive control protein
Immunogen:	GFP from the jellyfish Aequorea Victoria N-terminal peptide-KLH conjugates
Specificity:	Recognizes native and denatured forms of GFP and its variants such as: EGFP, YFP, EYFP, and CFP
Purification:	Protein A affinity chromatography from mouse ascites fluid
Applications:	Dot, ELISA, IP, IS, WB
Sensitivity:	WB (with ECL): 1-10ng of purified GFP or GFP fusion proteins
Working Condition:	WB (with ECL): 1:1000-3000 dilution (incubate for 1 hour at room temperature) IS: 1:500-2000 dilution For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test
Storage:	Store at -20°C for up to 2 years. Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 50ug/50ul. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for 1-2 weeks
Images:	Fig. 1:1000 (1µg/mL) Ab dilution probed against HEK293 cells transfected with GFP-tagged protein vector: untransfected control (1), 1µg (2) and 10µg (3) of cell lysates used