Anti-V5 Monoclonal Antibody

$150.00

Calatog Number:	LT9992
Lot Number:	411005100208
Isotype:	Mouse IgG1
Clonality:	E10
Quantity:	50 μg, 1μg/μl
Immunogen:	GKPIPNPLLGLDST (V5) synthetic peptide conjugated to KLH
Specificity:	Recognizes V5 (GKPIPNPLLGLDST) tagged fusion proteins
Purification:	Protein A affinity chromatography from culture supernatant of hybridoma cells grown in a bioreactor
Applications:	Dot, ELISA, IP, IS, WB
Sensitivity:	WB (with ECL): 1-2ng of V5-tagged fusion proteins
Working Condition:	WB (with ECL): 1:1000-3000 dilution (incubate for 1 hour at room temperature) IS: 1:500-2000 dilution For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test
Storage:	Store at -20°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for 1-2 weeks
Images:	Fig. 1:000 (1µg/mL) Ab dilution probed against HEK293 cells transfected with V5-tagged protein vector; untransfected (1) and transfected (2)

• 47 Units in Stock