Anti GST Monoclonal Antibody

Calatog Number:
LT0423
Lot Number:
411005100205
Isotype:
Mouse IgG2a
Clonality:
GST.B6
Quantity:
50 μg
Immunogen:
GST protein
Specificity:
Recognizes native and denatured forms of purified GST or GST fusion proteins
Purification:
Protein A affinity chromatography from mouse ascites fluid
Applications:
Dot, ELISA, IS, IP, WB
Sensitivity:
WB (with ECL): 10-50ng of purified GST or GST fusion proteins
Working Condition:
WB (with ECL): 1:1000-3000 dilution (incubate for 1 hour at room temperature)
IS: 1:500-2000 dilution
For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test
Storage:
Store at -20°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for 1-2 weeks
Images:
anti-GST mAb dilution probed against BL-21 bacteria lysate expressing the GST protein
Fig. 1:5000 (0.2µg/mL) Ab dilution probed against BL-21 bacteria lysate expressing the GST protein
  • 47 Units in Stock

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