LifeTein provides recombinant protein expression in mammalian systems, including HEK293 and CHO expression platforms, for projects that require correct folding, mammalian post-translational modification, and native biological activity.
This service is used for recombinant proteins, secreted proteins, and antibody expression projects where the target depends on eukaryotic expression for correct performance in downstream functional or structural studies.
| Expression systems | HEK293 and CHO mammalian expression platforms |
| Typical use | Proteins requiring native folding, secretion, post-translational modification, or antibody expression |
| Workflow | Gene synthesis, plasmid preparation, transient expression, purification, and recombinant protein delivery |
| Expression modes | Transient expression and project-appropriate production workflows |
| Location | New Jersey, USA |
Mammalian protein expression work is carried out in LifeTein’s New Jersey, USA facility, providing a clear domestic workflow for projects where location matters.
LifeTein supports codon optimization, gene synthesis, plasmid preparation, mammalian expression, purification, and delivery of purified recombinant protein.
Suspension culture expression in HEK293 or CHO systems can be selected according to the target protein and project needs.
The service also supports recombinant monoclonal antibody expression workflows, including projects starting from antibody sequence or hybridoma-derived variable regions.
Mammalian expression is particularly important when experimental performance depends on correct folding, native epitope presentation, secretion behavior, or appropriate post-translational modification. In these cases, bacterial expression may not be sufficient even if it is faster or less expensive.
LifeTein also supports hybridoma sequencing or antibody-sequence-based projects for recombinant monoclonal antibody expression. Variable-region sequences can be incorporated into mammalian expression vectors for transient or other project-appropriate antibody production workflows.
Need a faster bacterial option?
For rapid and cost-efficient protein production when mammalian post-translational modification is not required, see our Bacterial Protein Expression Service.
How long does a mammalian protein expression project usually take?
Project timing depends on whether gene synthesis, plasmid preparation, expression optimization, purification, and characterization are included. Mammalian workflows are typically chosen when the biological quality of the protein is more important than maximum speed.
Why choose mammalian expression instead of bacterial expression?
Mammalian systems are used when the target requires correct post-translational modification, secretion, or native folding for the intended assay or biological function.
What materials can I send to start a project?
You can start with a protein sequence, gene sequence, plasmid, antibody sequence, or hybridoma-related material, depending on the scope of the project.
Email your protein expression inquiry to sales@lifetein.com. We will send you a formal quote within 24 hours.