| LT0422 |
| 411005100206 |
| Mouse IgG3 |
| HA.C5 |
| 50 μg, lyophilized with PBS, pH7.4 |
| Purchase matching Western Blot positive control protein |
| YPYDVPDYA (HA) synthetic peptide conjugated to KLH |
| Recognizes the N-terminal, C-terminal or internal HA- tagged fusion proteins |
| Protein A affinity chromatography from mouse ascites fluid |
| Dot. ELISA, IP, IS, WB |
| WB (with ECL): 1-5ng of HA-tagged fusion proteins |
| WB (with ECL): 1:1000-3000 dilution (incubate for 1 hour at room temperature)
IS: 1:500-2000 dilution
For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test |
| Store at -20°C for up to 2 years. Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 50ug/50ul. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for 1-2 weeks |
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Fig. 1:1000 (1µg/mL) Ab dilution probed against HEK293 cells transfected with HA-tagged protein vector; untransfected (1) and transfected (2)
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YH Chou, G Pogorelko - Plant Physiology, 2012
...membrane (0.2 μm, Bio-Rad) for immunodetection. Monoclonal anti-GFP antibodies (MMS- 118P,Covance) were used (1:5000 dilution) for detection of YFP. Monoclonal anti-HA antibodies (LT0422, LifeTein) were used (1:500 dilution) to detect HA-fused proteins. Page 21. 21 ...
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