| LT9997 |
| 411005100213 |
| Mouse IgG1 |
| E32 |
| 50 μg |
| Recombinant full length ERK1 protein |
| Recognizes only the ERK1 protein (e.g.: from human and mouse cells) |
| WB: ERK1 from human and mouse cells. IP: ERK1 from human cells. ERK1 from other species may also be detectable |
| Protein A affinity chromatography from mouse ascites fluid |
| IP, WB |
| WB (with ECL): 1:1000-10,000 dilution (incubate for one hour at room temperature)
For best results with other assays (e.g.: IP, etc), please determine optimal working dilution by titration test |
| Store at -20°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for 1-2 weeks |
|  
Fig. LEFT: 1:1,000 (1µg/mL) Ab dilution used in WB of HEK293 cell lysate; 5µg (1), 10µg (2), and 30µg (3) of cell lysate used; RIGHT: IP of anti-ERK1 (1µL) using HeLa cell lysate; 10µg (1), 25µg (2), and 50µg (3) of cell lysate used |
