Anti HA Monoclonal Antibody

Catalog Number:
LT0422
Lot Number:
411005100206
Isotype:
Mouse IgG3
Clonality:
HA.C5
Quantity:
50 μg, lyophilized with PBS, pH7.4
Positive Control:
Purchase matching Western Blot positive control protein
Immunogen:
YPYDVPDYA (HA) synthetic peptide conjugated to KLH
Specificity:
Recognizes the N-terminal, C-terminal or internal HA- tagged fusion proteins
Purification:
Protein A affinity chromatography from mouse ascites fluid
Applications:
Dot. ELISA, IP, IS, WB
Sensitivity:
WB (with ECL): 1-5ng of HA-tagged fusion proteins
Working Condition:
WB (with ECL): 1:1000-3000 dilution (incubate for 1 hour at room temperature)
IS: 1:500-2000 dilution
For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test
Storage:
Store at -20°C for up to 2 years. Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 50ug/50ul. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for 1-2 weeks
Images:
anti-HA mAb dilution probed against HEK293 cells transfected with HA-tagged protein vector
Fig. 1:1000 (1µg/mL) Ab dilution probed against HEK293 cells transfected with HA-tagged protein vector; untransfected (1) and transfected (2)

Citations:

Xyloglucan xylosyltransferases XXT1, XXT2, XXT5, and the glucan synthase CSLC4 form Golgi-localized multiprotein complexes

YH Chou, G Pogorelko - Plant Physiology, 2012

...membrane (0.2 μm, Bio-Rad) for immunodetection. Monoclonal anti-GFP antibodies (MMS- 118P,Covance) were used (1:5000 dilution) for detection of YFP. Monoclonal anti-HA antibodies (LT0422, LifeTein) were used (1:500 dilution) to detect HA-fused proteins. Page 21. 21 ...

  • 20 Units in Stock

Ask a Question

$180.00

Add to Cart:

Customers who bought this product also purchased...

LifeTein LifeTein provides custom peptide synthesis service, recombinant proteins, peptides, cytokines, custom antibody service and custom protein service. LifeTein is the world leader in fast peptide synthesis service with lab facility located in New Jersey USA. LifeTein Peptide 100 Randolph Road, Suite 2D, Somerset USA New Jersey 08873
Copyright © 2024 LifeTein.com. Powered by LifeTein