Biotinylated Cynomolgus MSLN/Mesothelin Protein (Primary Amine Labeling) (LTP10699)

Product Name
Biotinylated Cynomolgus MSLN/Mesothelin Protein (Primary Amine Labeling) (LTP10699)
Catalog Number
LTP10699
Quantity
100ug
Price
$789 In stock
Background
Mesothelin, also known as MSLN, is a protein that in humans is encoded by the MSLN gene.Cloning studies showed that the mesothelin gene encodes a precursor protein that is processed to yield mesothelin which is attached to the cell membrane by a glycophosphatidylinositol linkage and a 31-kDa shed fragment named megakaryocyte-potentiating factor (MPF). Although it has been proposed that mesothelin may be involved in cell adhesion, its biological function is not known.A knockout mouse line that lacks mesothelin reproduces and develops normally.
Description
Recombinant Biotinylated Cynomolgus MSLN/Mesothelin Protein (Primary Amine Labeling) is expressed from Expi293 with His tag at the C-terminal. It contains Asp296-Gly580.
Target
MSLN/Mesothelin
Species
Cynomolgus
Gene/Protein ID
A0A2K5TW94-1
Exact Sequence
Asp296-Gly580
Molecular Weight
The protein has a predicted MW of 33 kDa. Due to glycosylation, the protein migrates to 35-50 kDa based on the Tris-Bis PAGE result.
Activity
Immobilized Anti-MSLN Antibody, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Cynomolgus MSLN, His Tag with the EC50 of 54.8ng/ml determined by ELISA. See testing image for detail.
Tag
C-His
Express System
Expi293
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization.
Stability and Storage

Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Shipping
Shipped at ambient temperature.
Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

Image
Biotinylated Cynomolgus MSLN/Mesothelin Protein (Primary Amine Labeling) (LTP10699)
  • 5 Units in Stock

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