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| Biotinylated Human 4-1BB/TNFRSF9 Protein (LTP10878) |
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| LTP10878 |
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| 100ug |
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$789 In stock |
| 4-1BB, is also known as CD137, is a type 2 transmembrane glycoprotein receptor belonging to the TNF superfamily.CD137 can be expressed by activated T cells, but to a larger extent on CD8 than on CD4 T cells. In addition, CD137 expression is found on dendritic cells, B cells, follicular dendritic cells, natural killer cells, granulocytes and cells of blood vessel walls at sites of inflammation. |
| Recombinant Biotinylated Human 4-1BB/TNFRSF9 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Leu24-Gln186. |
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| 4-1BB/TNFRSF9 |
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| Human |
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| Q07011 |
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| Leu24-Gln186 |
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| The protein has a predicted MW of 20.2 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on the Tris-Bis PAGE result. |
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| Immobilized Human 4-1BB Ligand Trimer, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human 4-1BB, His Tag with the EC50 of 0.58ug/ml determined by ELISA. See testing image for detail. |
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| C-His-Avi |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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