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| Biotinylated Human AFP (HLA-A*02:01) Protein (LTP10058) |
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| LTP10058 |
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| 100ug |
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$609 In stock |
| Alpha-fetoprotein (AFP), a specific liver cancer marker, T cells expressing AFP-CAR selectively degranulated, released cytokines, and lysed liver cancer cells that were HLA-A*02:01 /AFP while sparing cells from multiple tissue types that were negative for either expressed proteins.CAR T-cell immunotherapy targeting intracellular/secreted solid tumor antigens can elicit a potent antitumor response. |
| Recombinant Biotinylated Human AFP (HLA-A*02:01) Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and FMNKFIYEI peptide. |
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| AFP (HLA-A*02:01) |
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| Human |
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| A0A140T913(HLA-A*02:01)&P61769(B2M)&FMNKFIYEI |
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| Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M)and FMNKFIYEI peptide |
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| The protein has a predicted MW of 50.6 kDa. Due to glycosylation, the protein migrates to 53-63 kDa based on the Tris-Bis PAGE result. |
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| Immobilized Anti-AFP (HLA-A*02:01) Antibody, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human AFP (HLA-A*02:01) , His Tag with the EC50 of 0.19ug/ml determined by ELISA. See testing image for detail. |
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| C-His-Avi |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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