|
| Biotinylated Human ANGPT2/Angiopoietin-2 Protein (LTP11052) |
|
| LTP11052 |
|
| 100ug |
|
|
$789 In stock |
| Angiopoietin-2 (Ang-2; also ANGPT2) is a secreted glycoprotein that plays a complex role in angiogenesis and inflammation. Mature Ang-2 is 478 amino acids in length.Ang2 is widely expressed during development, but it is restricted postnatally to highly angiogenic tissues such as the placenta, ovaries, and uterus. It is particularly abundant in vascular endothelial cells (EC) where it is stored in intracellular Weibel Palade bodies. |
| Recombinant Biotinylated Human ANGPT2/Angiopoietin-2 Protein is expressed from Expi293 with His tag and Avi tag at the N-terminal. It contains Lys275-Phe496. |
|
| ANGPT2/Angiopoietin-2 |
|
| Human |
|
| O15123-1 |
|
| Lys275-Phe496 |
|
| The protein has a predicted MW of 28.4 kDa. Due to glycosylation, the protein migrates to 32-37 kDa based on the Tris-Bis PAGE result. |
|
| Immobilized Anti-ANGPT2 Antibody, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human ANGPT2, His Tag with the EC50 of 0.12ug/ml determined by ELISA. See testing image for detail. |
|
| N-His-Avi |
|
| Expi293 |
|
| > 95% as determined by Tris-Bis PAGE |
|
| Less than 1EU per ug by the LAL method. |
|
| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization. |
|
| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
| Shipped at ambient temperature. |
|
| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
|
|  |
