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| Biotinylated Human APRIL/TNFSF13 Trimer Protein (LTP10038) |
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| LTP10038 |
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| 100ug |
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$789 In stock |
| The APRIL (a proliferation-inducing ligand), also known as TNFSF13, TALL2, TRDL1, and CD256, is a member of the TNF ligand superfamily.Both APRIL and its close relative BAFF bind and signal through the TNF superfamily receptors TACI and BCMA, while BAFF additionally functions through BAFF R. |
| Recombinant Biotinylated Human APRIL/TNFSF13 Trimer Protein is expressed from Expi293 with His tag, Avi tag and Flag Tag at the N-terminal. It contains Lys112-Leu250. |
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| APRIL/TNFSF13 Trimer |
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| Human |
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| O75888-1 |
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| Lys112-Leu250 |
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| The protein has a predicted MW of 52.1 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on the Tris-Bis PAGE result. |
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| Immobilized Human BCMA, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human APRIL (Trimer) , His Tag with the EC50 of 16.3ng/ml determined by ELISA. See testing image for detail. |
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| N-His-Avi,N-Flag |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in 20mM PBS (pH 7.4). Normally 8% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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