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| Biotinylated Human B7-1/CD80 Protein (LTP10663) |
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| LTP10663 |
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| 100ug |
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$789 In stock |
| Cluster of differentiation 80 (also CD80 and B7-1) is a protein found on dendritic cells, activated B cells and monocytes that provides a costimulatory signal necessary for T cell activation and survival. It is the ligand for two different proteins on the T cell surface: CD28 and CTLA-4. |
| Recombinant Biotinylated Human B7-1/CD80 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Val35-Asn242. |
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| B7-1/CD80 |
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| Human |
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| P33681-1 |
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| Val35-Asn242 |
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| The protein has a predicted MW of 26.8 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on the Tris-Bis PAGE result. |
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| Immobilized Human CTLA-4, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human B7-1, His Tag with the EC50 of 1.9ug/ml determined by ELISA. See testing image for detail. |
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| C-His-Avi |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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