Biotinylated Human B7-1/CD80 Protein (LTP10663)

Product Name
Biotinylated Human B7-1/CD80 Protein (LTP10663)
Catalog Number
LTP10663
Quantity
100ug
Price
$789 In stock
Background
Cluster of differentiation 80 (also CD80 and B7-1) is a protein found on dendritic cells, activated B cells and monocytes that provides a costimulatory signal necessary for T cell activation and survival. It is the ligand for two different proteins on the T cell surface: CD28 and CTLA-4.
Description
Recombinant Biotinylated Human B7-1/CD80 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Val35-Asn242.
Target
B7-1/CD80
Species
Human
Gene/Protein ID
P33681-1
Exact Sequence
Val35-Asn242
Molecular Weight
The protein has a predicted MW of 26.8 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on the Tris-Bis PAGE result.
Activity
Immobilized Human CTLA-4, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human B7-1, His Tag with the EC50 of 1.9ug/ml determined by ELISA. See testing image for detail.
Tag
C-His-Avi
Express System
Expi293
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization.
Stability and Storage

Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Shipping
Shipped at ambient temperature.
Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

Image
Biotinylated Human B7-1/CD80 Protein (LTP10663)
  • 5 Units in Stock

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