|
| Biotinylated Human B7-H3 (4Ig) /B7-H3b Protein (LTP11096) |
|
| LTP11096 |
|
| 100ug |
|
|
$789 In stock |
| B7-H3, a member of the B7 family of immunomodulatory molecules, is overexpressed in a wide range of solid cancers.B7-H3 binds to activated T cells via an as yet unidentified receptor. In assays using sub-optimal amount so anti-CD3 stimulation, 2Ig?B7?H3 enhances T cell proliferation, T cell interferon-gamma (IFN-gamma) production, and cytotoxic T cells induction. |
| Recombinant Biotinylated Human B7-H3 (4Ig)/B7-H3b Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Gly27-Thr461. |
|
| B7-H3 (4Ig)/B7-H3b |
|
| Human |
|
| Q5ZPR3-1 |
|
| Gly27-Thr461 |
|
| The protein has a predicted MW of 49.5 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on the Tris-Bis PAGE result. |
|
| Immobilized Anti-B7-H3 Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human B7-H3 (4Ig) , His Tag with the EC50 of 7.7ng/ml determined by ELISA. See testing image for detail. |
|
| C-His-Avi |
|
| Expi293 |
|
| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
|
| Less than 1EU per ug by the LAL method. |
|
| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization. |
|
| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
| Shipped at ambient temperature. |
|
| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
|
|  |
