Biotinylated Human CD24 Protein (Primary Amine Labeling) (LTP10585)

Product Name
Biotinylated Human CD24 Protein (Primary Amine Labeling) (LTP10585)
Catalog Number
LTP10585
Quantity
100ug
Price
$789 In stock
Background
CD24 is a sialoglycoprotein expressed at the surface of most B lymphocytes and differentiating neuroblasts. It is also expressed on neutrophils and neutrophil precursors from the myelocyte stage onwards. The potential for targeting CD24 in cancer therapy seems promising, as CD24 is overexpressed in many human cancers.
Description
Recombinant Biotinylated Human CD24 Protein (Primary Amine Labeling) is expressed from Expi293 with mFc (IgG1) tag at the C-terminal. It contains Ser27-Gly59.
Target
CD24
Species
Human
Gene/Protein ID
P25063-1
Exact Sequence
Ser27-Gly59
Molecular Weight
The protein has a predicted MW of 29.5 kDa. Due to glycosylation, the protein migrates to 48-58 kDa based on the Tris-Bis PAGE result.
Activity
Immobilized Anti-CD24 Antibody at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human CD24, mFc Tag with the EC50 of 0.83ug/ml determined by ELISA. See testing image for detail.
Tag
C-mFc
Express System
Expi293
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization.
Stability and Storage

Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Shipping
Shipped at ambient temperature.
Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

Image
Biotinylated Human CD24 Protein (Primary Amine Labeling) (LTP10585)
  • 5 Units in Stock

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