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| Biotinylated Human CD3E/CD3 epsilon 1-27 peptide Protein (LTP10581) |
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| LTP10581 |
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| 100ug |
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$900 In stock |
| CD3E, is a single-pass type I membrane protein.CD3 (cluster of differentiation 3) T cell co-receptor helps to activate both the cytotoxic T cell (CD8 naive T cells) and also T helper cells (CD4 naive T cells). It consists of a protein complex and is composed of four distinct chains. In mammals, the complex contains a CD3? chain, a CD3? chain, and two CD3?u chains. |
| Recombinant Biotinylated Human CD3E/CD3 epsilon 1-27 peptide Protein is expressed from Expi293 with hFc tag and Avi Tag at the C-terminal. It contains Asp23-Thr48. |
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| CD3E/CD3 epsilon 1-27 peptide |
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| Human |
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| P07766 |
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| Asp23-Thr48 |
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| The protein has a predicted MW of 31.3 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on the Tris-Bis PAGE result. |
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| Immobilized Anti-CD3 Antibody, mFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD3E 1-27 peptide, hFc Tag with the EC50 of 5.9ng/ml determined by ELISA. See testing image for detail. |
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| C-hFc-Avi |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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