Biotinylated Human CD40/TNFRSF5 Protein (LTP10783)

Product Name
Biotinylated Human CD40/TNFRSF5 Protein (LTP10783)
Catalog Number
LTP10783
Quantity
100ug
Price
$789 In stock
Background
CD40 is a costimulatory protein found on antigen presenting cells and is required for their activation. The binding of CD154 (CD40L) on TH cells to CD40 activates antigen presenting cells and induces a variety of downstream effects.CD40 molecule is a potential target for cancer immunotherapy. There are number of completed and ongoing clinical trials where agonistic anti-CD40 monoclonal antibodies are employed to activate an anti-tumor T cell response via activation of dendritic cells.
Description
Recombinant Biotinylated Human CD40/TNFRSF5 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Glu21-Arg193.
Target
CD40/TNFRSF5
Species
Human
Gene/Protein ID
P25942
Exact Sequence
Glu21-Arg193
Molecular Weight
The protein has a predicted MW of 22.1 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on the Tris-Bis PAGE result.
Activity
Immobilized Biotinylated Human CD40, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human CD40L, hFc Tag with the EC50 of 33ng/ml determined by ELISA. See testing image for detail.
Tag
C-His-Avi
Express System
Expi293
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization.
Stability and Storage

Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Shipping
Shipped at ambient temperature.
Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

Image
Biotinylated Human CD40/TNFRSF5 Protein (LTP10783)
  • 5 Units in Stock

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