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| Biotinylated Human CD45/PTPRC Protein (LTP10497) |
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| LTP10497 |
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| 100ug |
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$789 In stock |
| PTPRC (also known as CD45),T cells require the protein tyrosine phosphatase CD45 to detect and respond to antigen because it activates the Src family kinase Lck, which phosphorylates the T cell antigen receptor (TCR) complex. CD45 ativates Lck by opposing the negative regulatory kinase Csk. Paradoxically, CD45 has also been implicated in suppressing TCR signaling by dephosphorylating the same signaling motifs within the TCR complex upon which Lck acts. |
| Recombinant Biotinylated Human CD45/PTPRC Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Gln26-Lys416. |
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| CD45/PTPRC |
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| Human |
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| P08575-4 |
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| Gln26-Lys416 |
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| The protein has a predicted MW of 47.3 kDa. Due to glycosylation, the protein migrates to 80-110 kDa based on the Tris-Bis PAGE result. |
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| Immobilized Anti-CD45 Antibody at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD45, His Tag with the EC50 of 0.16ug/ml determined by ELISA. See testing image for detail. |
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| C-His-Avi |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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