Biotinylated Human CDCP1 Protein (LTP10409)

Product Name
Biotinylated Human CDCP1 Protein (LTP10409)
Catalog Number
LTP10409
Quantity
100ug
Price
$789 In stock
Background
Tumor metastasis depends on the dynamic regulation of cell adhesion through ?1-integrin. The Cub-Domain Containing Protein-1, CDCP1, is a transmembrane glycoprotein which regulates cell adhesion. Overexpression and loss of CDCP1 have been observed in the same cancer types to promote metastatic progression.
Description
Recombinant Biotinylated Human CDCP1 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Phe30-Leu666.
Target
CDCP1
Species
Human
Gene/Protein ID
Q9H5V8-1
Exact Sequence
Phe30-Leu666
Molecular Weight
The protein has a predicted MW of 74.6 kDa. Due to glycosylation, the protein migrates to 85-100 kDa based on the Tris-Bis PAGE result.
Activity
Immobilized Anti-CDCP1 Antibody, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human CDCP1, His Tag with the EC50 of 64.8ng/ml determined by ELISA. See testing image for detail.
Tag
C-His-Avi
Express System
Expi293
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization.
Stability and Storage

Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Shipping
Shipped at ambient temperature.
Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

Image
Biotinylated Human CDCP1 Protein (LTP10409)
  • 5 Units in Stock

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