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| Biotinylated Human CEACAM-5/CD66e Protein (LTP11014) |
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| LTP11014 |
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| 100ug |
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$789 In stock |
| Carcinoembryonic antigen-related cell adhesion molecule 5 (CEACAM5) was identified as a metastatic driver. CEACAM5 overproduction enriched for an epithelial gene expression pattern and facilitated tumor outgrowth at metastatic sites. Tissues from patients with metastatic breast cancer confirmed elevated levels of CEACAM5 in lung metastases relative to breast tumors, and an inverse correlation between CEACAM5 and the mesenchymal marker vimentin was demonstrated. |
| Recombinant Biotinylated Human CEACAM-5/CD66e Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Lys35-Ala685. |
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| CEACAM-5/CD66e |
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| Human |
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| Q8N4D0 |
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| Lys35-Ala685 |
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| The protein has a predicted MW of 74.2 kDa. Due to glycosylation, the protein migrates to 115-145 kDa based on the Tris-Bis PAGE result. |
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| Immobilized Anti-CEACAM-5 Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CEACAM-5, His Tag with the EC50 of 26.3ng/ml determined by ELISA. See testing image for detail. |
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| C-His-Avi |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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