Biotinylated Human CTGF/CCN2 Protein (LTP10195)

Product Name
Biotinylated Human CTGF/CCN2 Protein (LTP10195)
Catalog Number
LTP10195
Quantity
100ug
Price
$789 In stock
Background
Connective tissue growth factor (CTGF) is a member of the CCN matricellular protein family, consisting of four domains, that regulates the signaling of other growth factors and promotes kidney fibrosis.CTGF can simultaneously interact with several factors with its four domains. The microenvironment differs depending on the types of cells and tissues and differentiation stages of these cells.
Description
Recombinant Biotinylated Human CTGF/CCN2 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Gln27-Ala349.
Target
CTGF/CCN2
Species
Human
Gene/Protein ID
Q5M8T4-1
Exact Sequence
Gln27-Ala349
Molecular Weight
The protein has a predicted MW of 38.4 kDa. Due to glycosylation, the protein migrates to 43-53 kDa based on the Tris-Bis PAGE result.
Activity
Immobilized Anti-CTGF Antibody at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CTGF, His Tag with the EC50 of 39.9ng/ml determined by ELISA. See testing image for detail.
Tag
C-His-Avi
Express System
Expi293
Purity
> 95% as determined by Tris-Bis PAGE
Endotoxin
Less than 1EU per ug by the LAL method.
Formulation
Lyophilized from 0.22 um filtered solution in PBS, 200mM L-Arginine (pH 7.4). Normally 8% trehalose is added as a protectant before lyophilization.
Stability and Storage

Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Shipping
Shipped at ambient temperature.
Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

Image
Biotinylated Human CTGF/CCN2 Protein (LTP10195)
  • 5 Units in Stock

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