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| Biotinylated Human CTLA-4/CD152 Protein (LTP11016) |
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| LTP11016 |
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| 100ug |
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$450 In stock |
| CTLA-4 (cytotoxic T-lymphocyte-associated protein 4), also known as CD152, is a protein receptor that, functioning as an immune checkpoint, downregulates immune responses.CTLA4 is constitutively expressed in regulatory T cells but only upregulated in conventional T cells after activation – a phenomenon which is particularly notable in cancers. It acts as an "off" switch when bound to CD80 or CD86 on the surface of antigen-presenting cells. |
| Recombinant Biotinylated Human CTLA-4 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Lys36-Asp161. |
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| CTLA-4/CD152 |
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| Human |
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| P16410 |
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| Lys36-Asp161 |
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| The protein has a predicted MW of 16.3 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on the Tris-Bis PAGE result. |
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| Immobilized Anti-CTLA-4 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CTLA-4, His Tag with the EC50 of 45.7ng/ml determined by ELISA (QC Test). See testing image for detail. |
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| C-His-Avi |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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