Biotinylated Human EGFRVIII Protein (LTP10743)

Product Name
Biotinylated Human EGFRVIII Protein (LTP10743)
Catalog Number
LTP10743
Quantity
100ug
Price
$789 In stock
Background
The epidermal growth factor receptor (EGFR) is overexpressed in a variety of human epithelial tumors, often as a consequence of gene amplification. Tumors with EGFR gene amplification frequently contain EGFR gene rearrangements, with the most common extracellular domain mutation being EGFRvIII. This mutation leads to a deletion of exons 2-7 of the EGFR gene and renders the mutant receptor incapable of binding any known ligand.
Description
Recombinant Biotinylated Human EGFRVIII Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Leu25-Ser378.
Target
EGFRVIII
Species
Human
Gene/Protein ID
NP_001333870.1
Exact Sequence
Leu25-Ser378
Molecular Weight
The protein has a predicted MW of 41.6 kDa. Due to glycosylation, the protein migrates to 60-78 kDa based on the Tris-Bis PAGE result.
Activity
Immobilized Anti-EGFR VIII Anitibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human EGFR VIII, His Tag with the EC50 of 56.1ng/ml determined by ELISA. See testing image for detail.
Tag
C-His-Avi
Express System
Expi293
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization.
Stability and Storage

Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Shipping
Shipped at ambient temperature.
Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

Image
Biotinylated Human EGFRVIII Protein (LTP10743)
  • 5 Units in Stock

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