|
| Biotinylated Human Fc gamma RIIIA/CD16a (F176) Protein (LTP11065) |
|
| LTP11065 |
|
| 100ug |
|
|
$789 In stock |
| Human Fc gamma RIIIA/CD16a Protein is a receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. |
| Recombinant Biotinylated Human Fc gamma RIIIA/CD16a (F176) Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.. It contains Gly17-Gln208(F176). |
|
| Fc gamma RIIIA/CD16a (F176) |
|
| Human |
|
| P08637-1 |
|
| Gly17-Gln208(F176) |
|
| The protein has a predicted MW of 24.7 kDa. Due to glycosylation, the protein migrates to 48-58 kDa based on the Tris-Bis PAGE result. |
|
| Immobilized Anti-Fc gamma RIIIA Antibody, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human Fc gamma RIIIA (F176) , His Tag with the EC50 of 37.7ng/ml determined by ELISA (QC Test). The affinity constant of 0.14uM as determined in SPR assay (Biacore T200). See testing image for detail. |
|
| C-His-Avi |
|
| Expi293 |
|
| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
|
| Less than 1EU per ug by the LAL method. |
|
| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization. |
|
| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
| Shipped at ambient temperature. |
|
| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
|
|  |
