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| Biotinylated Human Fc gamma RIIIA/CD16a (V176) Protein (LTP11066) |
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| LTP11066 |
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| 100ug |
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$789 In stock |
| The Human Fc gamma RIIIA/CD16a Protein is a receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. |
| Recombinant Biotinylated Human Fc gamma RIIIA/CD16a (V176) Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Gly17-Gln208. |
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| Fc gamma RIIIA/CD16a (V176) |
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| Human |
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| AAH17865 |
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| Gly17-Gln208 |
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| The protein has a predicted MW of 24.7 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on the Tris-Bis PAGE result. |
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| Immobilized Anti-Fc gamma RIIIA Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Fc gamma RIIIA (V176) , His Tag with the EC50 of 2ng/ml determined by ELISA (QC Test). The affinity constant of 0.539 uM as determined in SPR assay (Biacore T200). See testing image for detail. |
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| C-His-Avi |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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