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| Biotinylated Human FGF21 Protein (LTP10062) |
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| LTP10062 |
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| 100ug |
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$789 In stock |
| Fibroblast growth factor 21 (FGF21) is a peptide hormone that is synthesized by several organs and regulates energy homeostasis. Excitement surrounding this relatively recently identified hormone is based on the documented metabolic beneficial effects of FGF21, which include weight loss and improved glycemia. |
| Recombinant Biotinylated Human FGF21 Protein is expressed from Expi293 with mFc (IgG1) tag and Avi tag at the N-terminal. It contains His29-Ser209. |
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| FGF21 |
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| Human |
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| Q9NSA1 |
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| His29-Ser209 |
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| The protein has a predicted MW of 46.9 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on the Tris-Bis PAGE result. |
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| Immobilized Biotinylated Human FGF21, mFc Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human Beta Klotho, His Tag with the EC50 of 0.26ug/ml determined by ELISA. See testing image for detail. |
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| N-mFc-Avi |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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