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| Biotinylated Human FGFR1 alpha (IIIc) Protein (LTP11051) |
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| LTP11051 |
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| 100ug |
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$789 In stock |
| Fibroblast growth factors (FGFs) comprise a family of at least eighteen structurally related proteins that are involved in a multitude of physiological and pathological cellular processes, including cell growth, differentiation, angiogenesis, wound healing and tumorgenesis. The biological activities of the FGFs are mediated by a family of type I transmembrane tyrosine kinases which undergo dimerization and autophosphorylation after ligand binding.FGFR1 is tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of embryonic development, cell proliferation, differentiation and migration. Required for normal mesoderm patterning and correct axial organization during embryonic development, normal skeletogenesis and normal development of the gonadotropin-releasing hormone (GnRH) neuronal system. |
| Recombinant Biotinylated Human FGFR1 alpha (IIIc) Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Arg22-Glu374. |
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| FGFR1 alpha (IIIc) |
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| Human |
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| P11362-7 |
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| Arg22-Glu374 |
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| The protein has a predicted MW of 42 kDa. Due to glycosylation, the protein migrates to 68-80 kDa based on the Tris-BisPAGE result. |
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| Immobilized Anti-FGFR1 Anitibody, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human FGFR1 alpha (IIIc) , His Tag with the EC50 of 25.9ng/ml determined by ELISA. See testing image for detail. |
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| C-His-Avi |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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