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| Biotinylated Human FGFR2 beta (IIIb) Protein (LTP10337) |
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| LTP10337 |
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| 100ug |
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$789 In stock |
| Four distinct genes encoding closely related FGF receptors, FGF R1 - 4, are known. All four genes for FGF Rs encode proteins with an N-terminal signal peptide, three immunoglobulin (Ig)-like domains, an acid-box region containing a run of acidic residues between the IgI and IgII domains, a transmembrane domain and the split tyrosine-kinase domain. Multiple forms of FGF R1 - 3 are generated by alternative splicing of the mRNAs. A frequent splicing event involving FGF R1 and 2 results in receptors containing all three Ig domains, referred to as the alpha isoform, or only IgII and IgIII, referred to as the beta isoform. |
| Recombinant Biotinylated Human FGFR2 beta (IIIb) Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Arg152-Glu378. |
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| FGFR2 beta (IIIb) |
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| Human |
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| P21802-3 |
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| Arg152-Glu378 |
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| The protein has a predicted MW of 28.3 kDa. Due to glycosylation, the protein migrates to 48-60 kDa based on the Tris-Bis PAGE result. |
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| Immobilized Anti-FGFR2 IIIb Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human FGFR2 beta (IIIb) , His Tag with the EC50 of 13.1ng/ml determined by ELISA. See testing image for detail. |
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| C-His-Avi |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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