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| Biotinylated Human FGFR3 alpha (IIIb) Protein (LTP11023) |
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| LTP11023 |
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| 100ug |
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$789 In stock |
| Four distinct genes encoding closely related FGF receptors, FGF R1-4, are known. All four genes for FGF Rs encode proteins with an N-terminal signal peptide, three immunoglobulin (Ig)-like domains, an acid?box region containing a run of acidic residues between the IgI and IgII domains, a transmembrane domain and the split tyrosine-kinase domain.FGFR3 is tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation and apoptosis. Plays an essential role in the regulation of chondrocyte differentiation, proliferation and apoptosis, and is required for normal skeleton development. Regulates both osteogenesis and postnatal bone mineralization by osteoblasts. |
| Recombinant Biotinylated Human FGFR3 (IIIb) Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Glu23-Gly377. |
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| FGFR3 alpha (IIIb) |
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| Human |
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| P22607-2 |
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| Glu23-Gly377 |
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| The protein has a predicted MW of 41.4 kDa. Due to glycosylation, the protein migrates to 65-75 kDa based on the Tris-Bis PAGE result. |
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| Immobilized Biotinylated Human FGFR3 alpha (lllb) at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-FGFR3 Antibody, hFc Tag with the EC50 of 11.9ng/ml determined by ELISA. See testing image for detail. |
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| C-His-Avi |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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