|
| Biotinylated Human GDF15 Protein (Primary Amine Labeling) (LTP10538) |
|
| LTP10538 |
|
| 100ug |
|
|
$1013 In stock |
| Growth and differentiation factor 15 (GDF15) is an inflammation-associated hormone with poorly defined biology. Here, we investigated the role of GDF15 in bacterial and viral infections. Inflammation induced GDF15, and that GDF15 was necessary for surviving both bacterial and viral infections, as well as sepsis. The protective effects of GDF15 were largely independent of pathogen control or the magnitude of inflammatory response, suggesting a role in disease tolerance. |
| Recombinant Biotinylated Human GDF15 Protein (Primary Amine Labeling) is expressed from Expi293 with hFc tag at the N-terminal. It contains Ala197-Ile308. |
|
| GDF15 |
|
| Human |
|
| Q99988 |
|
| Ala197-Ile308 |
|
| The protein has a predicted MW of 37.9 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on the Tris-Bis PAGE result. |
|
| Immobilized Human GFRAL, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human GDF15, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA. See testing image for detail. |
|
| N-hFc |
|
| Expi293 |
|
| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
|
| Less than 1EU per ug by the LAL method. |
|
| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization. |
|
| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
| Shipped at ambient temperature. |
|
| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
|
|  |
