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| Biotinylated Human GFRAL/GFR alpha-like Protein (LTP10546) |
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| LTP10546 |
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| 100ug |
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$789 In stock |
| GFR alpha -like (GDNF receptor-alpha-like) is a distant member of the GDNFR family of proteins. Mature human GFR alpha-like is a 376 amino acid (aa) type I transmembrane protein. It contains a 333 aa extracellular domain, a 20 aa transmembrane domain and a 23 aa cytoplasmic domain.GFRAL is a brainstem-restricted receptor for GDF15 which regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses. |
| Recombinant Biotinylated Human GFRAL/GFR alpha-like Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Ser19-Glu351. |
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| GFRAL/GFR alpha-like |
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| Human |
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| Q6UXV0 |
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| Ser19-Glu351 |
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| The protein has a predicted MW of 40.7 kDa. Due to glycosylation, the protein migrates to 48-62 kDa based on the Tris-Bis PAGE result. |
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| Immobilized Human GDF15, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human GFRAL, His Tag with the EC50 of 0.1ug/ml determined by ELISA. See testing image for detail. |
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| C-His-Avi |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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