Biotinylated Human Her3/ErbB3 Protein (LTP11087)

Product Name
Biotinylated Human Her3/ErbB3 Protein (LTP11087)
Catalog Number
LTP11087
Quantity
100ug
Price
$789 In stock
Background
Her3, also called ErbB3, is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors.Her3 is expressed in keratinocytes, melanocytes, skeletal muscle cells, embryonic myoblasts and Schwann cells. Monomeric Her3 serves as a low affinity receptor for the heregulins (HRG).ation leads to a deletion of exons 2-7 of the EGFR gene and renders the mutant receptor incapable of binding any known ligand.
Description
Recombinant Biotinylated Human Her3/ErbB3 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Ser20-Thr643.
Target
Her3/ErbB3
Species
Human
Gene/Protein ID
P21860-1
Exact Sequence
Ser20-Thr643
Molecular Weight
The protein has a predicted MW of 71.6 kDa. Due to glycosylation, the protein migrates to 90-100 kDa based on the Tris-Bis PAGE result.
Activity
Immobilized Anti-Her3 Antibody, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylatd Human Her3, His Tag with the EC50 of 58.1ng/ml determined by ELISA. See testing image for detail.
Tag
C-His-Avi
Express System
Expi293
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as a protectant before lyophilization.
Stability and Storage

Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Shipping
Shipped at ambient temperature.
Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

Image
Biotinylated Human Her3/ErbB3 Protein (LTP11087)
  • 5 Units in Stock

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