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| Biotinylated Human HGF R/c-MET Protein (LTP10398) |
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| LTP10398 |
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| 100ug |
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$789 In stock |
| c-Met, also called tyrosine-protein kinase Met or hepatocyte growth factor receptor (HGF R), is a protein that in humans is encoded by the MET gene.The protein possesses tyrosine kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1. |
| Recombinant Biotinylated Human HGF R/c-MET Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Glu25-Thr932. |
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| HGF R/c-MET |
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| Human |
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| P08581-1 |
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| Glu25-Thr932 |
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| The mature form of HGF R is a heterodimer which can be cleaved into ? and ? chain. The protein has a predicted MW of 32.5 kDa (? chain) and 72.1 kDa (? chain). Due to glycosylation, the protein migrates to 45-50 kDa and 80-90 kDa based on the Tris-Bis PAGE result. |
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| Immobilized Anti-HGF R Antibody, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human HGF R, His Tag with the EC50 of 25.7ng/ml determined by ELISA. See testing image for detail. |
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| C-His-Avi |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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