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| Biotinylated Human IFN gamma/IFNG Protein (LTP11032) |
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| LTP11032 |
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| 100ug |
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$789 In stock |
| Interferon-gamma (IFN gamma) is a cytokine that plays physiologically important roles in promoting innate and adaptive immune responses. The absence of IFN gamma production or cellular responsiveness in humans and experimental animals significantly predisposes the host to microbial infection, a result that validates the physiologic importance of this cytokine in preventing infectious disease. |
| Recombinant Biotinylated Human IFN gamma/IFNG Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Gln24-Gly161. |
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| IFN gamma/IFNG |
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| Human |
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| P01579 |
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| Gln24-Gly161 |
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| The protein has a predicted MW of 19.1 kDa. Due to glycosylation, the protein migrates to 25-40 kDa based on the Tris-Bis PAGE result. |
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| Immobilized Human IFNGR1, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IFN gamma, His Tag with the EC50 of 39.1g/ml determined by ELISA. See testing image for detail. |
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| C-His-Avi |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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