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| Biotinylated Human IGF1R/CD221 Protein (LTP10691) |
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| LTP10691 |
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| 100ug |
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$789 In stock |
| The type 1 IGF receptor (IGF1R) is a transmembrane tyrosine kinase that is frequently overexpressed by tumours, and mediates proliferation and apoptosis protection. IGF signalling also influences hypoxia signalling, protease secretion, tumour cell motility and adhesion, and thus can affect the propensity for invasion and metastasis. Therefore, the IGF1R is now an attractive anti-cancer treatment target. |
| Recombinant Biotinylated Human IGF1R/CD221 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Glu31-Asn932. |
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| IGF1R/CD221 |
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| Human |
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| P08069 |
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| Glu31-Asn932 |
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| The protein has a predicted MW of 105.8 kDa (alpha subunit) and 23 kDa (beta subunit). Due to glycosylation, the protein migrates to 110-120 kDa (alpha subunit) and 52-55 kDa (beta subunit) based on the Tris-Bis PAGE result. |
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| Immobilized Anti-IGF1R Antibody at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IGF1R, His Tag with the EC50 of 48.0ng/ml determined by ELISA. See testing image for detail. |
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| C-His-Avi |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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