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| Biotinylated Human IL-12 Protein (LTP10540) |
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| LTP10540 |
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| 100ug |
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$1126 In stock |
| Interleukin 12 (IL-12) is a pleiotropic cytokine that plays an essential role in Th1-type immune response against cancer, a condition where cells in a particular part of the body grow and reproduce uncontrollably. |
| Recombinant Biotinylated Human IL-12 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Ile23-Ser328(IL-12B)/Arg23-Ser219(IL-12A). |
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| IL-12 |
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| Human |
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| P29460(IL-12B)&P29459(IL-12A) |
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| Ile23-Ser328(IL-12B)/Arg23-Ser219(IL-12A) |
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| The protein has a predicted MW of 34.7(IL-12B) kDa / 25.5 (IL-12A) kDa. Due to glycosylation, the protein migrates to 40-45 kDa based on the Tris-Bis PAGE result. |
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| Immobilized Anti-IL-12 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-12, His Tag with the EC50 of 13.0ng/ml determined by ELISA. See testing image for detail. |
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| C-His-Avi |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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