Biotinylated Human IL-15RA/IL-15 R alpha/CD215 Protein (LTP11057)

Product Name
Biotinylated Human IL-15RA/IL-15 R alpha/CD215 Protein (LTP11057)
Catalog Number
LTP11057
Quantity
100ug
Price
$789 In stock
Background
Interleukin-15 receptor alpha (IL-15R alpha) is a high affinity IL-15 binding protein that is crucial for mediating IL-15 functions such as memory CD8 T cell proliferation and NK, NK/T cell, and intestinal intraepithelial lymphocyte development.
Description
Recombinant Biotinylated Human IL-15RA/IL-15 R alpha/CD215 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Ile31-Thr205.
Target
IL-15RA/IL-15 R alpha/CD215
Species
Human
Gene/Protein ID
Q13261-1
Exact Sequence
Ile31-Thr205
Molecular Weight
The protein has a predicted MW of 21.3 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on the Tris-Bis PAGE result.
Activity
Immobilized Human IL-15 at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human IL-15RA, His Tag with the EC50 of 45.9ng/ml determined by ELISA. See testing image for detail.
Tag
C-His-Avi
Express System
Expi293
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as a protectant before lyophilization.
Stability and Storage

Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Shipping
Shipped at ambient temperature.
Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

Image
Biotinylated Human IL-15RA/IL-15 R alpha/CD215 Protein (LTP11057)
  • 5 Units in Stock

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