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| Biotinylated Human Integrin alpha V beta 8 (ITGAV&ITGB8) Heterodimer Protein (LTP10089) |
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| LTP10089 |
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| 100ug |
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$900 In stock |
| Deletions of the genes encoding the integrin ?V?8 (Itgav, Itgb8) have been shown to result in abnormal vascular development in the CNS, including prenatal and perinatal hemorrhage. Other work has indicated that a major function of this integrin in vivo is to promote TGF? activation. |
| Recombinant Biotinylated Human Integrin alpha V beta 8 (ITGAV&ITGB8) Heterodimer Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Phe31-Val992(ITGAV)&Glu43-Arg684(ITGB8). |
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| ITGAV&ITGB8 |
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| Human |
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| P06756-1(ITGAV)&P26012-1(ITGB8) |
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| Phe31-Val992(ITGAV) & Glu43-Arg684(ITGB8) |
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| The protein has a predicted MW of 114 kDa (ITGAV)&76 kDa (ITGB8) kDa. Due to glycosylation, the protein migrates to 85-145 kDa based on the Tris-Bis PAGE result. |
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| Immobilized Human Latent TGF beta 1, His Tag beta 1 at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human ITGAV&ITGB8, His Tag with the EC50 of 6.6ug/ml determined by ELISA. See testing image for detail. |
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| C-His-Avi |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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