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| Biotinylated Human KRAS G12V (HLA-A*03:01) Protein (LTP10001) |
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| LTP10001 |
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| 100ug |
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$609 In stock |
| Kirsten rat sarcoma 2 viral oncogene homolog (KRAS) is the most commonly mutated oncogene in human cancer. The developments of many cancers depend on sustained expression and signaling of KRAS, which makes KRAS a high-priority therapeutic target. The virtual screening approach to discover novel KRAS inhibitors and synthetic lethality interactors of KRAS are discussed in detail. |
| Recombinant Biotinylated Human KRAS G12V (HLA-A*03:01) Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Gly25-Thr305(HLA-A*03:01),Ile21-Met119(B2M) and VVVGAVGVGK peptide. |
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| KRAS G12V (HLA-A*03:01) |
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| Human |
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| NP_002107.3(HLA-A*03:01)&P61769(B2M)&VVGAVGVGK |
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| Gly25-Thr305(HLA-A*03:01), Ile21-Met119(B2M)and VVVGAVGVGK peptide |
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| The protein has a predicted MW of 50.2 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on the Tris-Bis PAGE result. |
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| The affinity constant of 0.14 uM as determined in SPR assay (Biacore T200). See testing image for detail. |
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| C-His-Avi |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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