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| Biotinylated Human LAP (TGF beta 1) Protein (LTP10668) |
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| LTP10668 |
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| 100ug |
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$900 In stock |
| Latent TGF beta 1 cDNA encodes a 390 amino acid precursor that contains a 29 aa signal peptide and a 361 aa proprotein. A furin?like convertase processes the proprotein to generate an N?terminal 249 aa latency?associated peptide (LAP) and a C?terminal 112 aa mature TGF? beta 1. Disulfide?linked homodimers of LAP and TGF? beta 1 remain non?covalently associated after secretion, forming the small latent TGF? beta 1 complex. |
| Recombinant Biotinylated Human LAP (TGF beta 1) Protein is expressed from Expi293 with His tag and Avi tag at the N-terminal. It contains Leu30-Arg278(C33S). |
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| LAP (TGF beta 1) |
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| Human |
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| P01137 |
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| Leu30-Arg278(C33S) |
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| The protein has a predicted MW of 31.4 kDa. Due to glycosylation, the protein migrates to 45-50 kDa based on the Tris-Bis PAGE result. |
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| Immobilized Human ITGAV&ITGB6, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human LAP (TGF beta 1) , His Tag with the EC50 of 0.86ug/ml determined by ELISA (QC Test). See testing image for detail. |
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| N-His-Avi |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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