| Biotinylated Human NKp30/NCR3/CD337 Protein (LTP10523) |
| LTP10523 |
| 100ug |
|
$789 In stock |
| NKp30, along with NKp44 and NKp46, constitute a group of receptors termed "Natural Cytotoxicity Receptors". These receptors play a major role in triggering NK-mediated killing of most tumor cells lines.NKp30 stimulates NK cells cytotoxicity toward neighboring cells producing these ligands. It controls, for instance, NK cells cytotoxicity against tumor cells. |
| Recombinant Biotinylated Human NKp30/NCR3/CD337 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Leu19-Thr138. |
| NKp30/NCR3/CD337 |
| Human |
| AAH52582 |
| Leu19-Thr138 |
| The protein has a predicted MW of 20 kDa. Due to glycosylation, the protein migrates to 26-45 kDa based on the Tris-Bis PAGE result. |
| Immobilized Anti-NKp30 Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human NKp30, His Tag with the EC50 of 30ng/ml determined by ELISA (QC Test). The affinity constant of 0.330 uM as determined in SPR assay (Biacore T200). See testing image for detail. |
| C-His-Avi |
| Expi293 |
| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
| Less than 1EU per ug by the LAL method. |
| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization. |
| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
| Shipped at ambient temperature. |
| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| |