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| Biotinylated Human PD-1/PDCD1 Protein (LTP10521) |
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| LTP10521 |
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| 100ug |
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$789 In stock |
| Programmed cell death protein 1, also known as PD-1 and CD279 , is a protein found on the surface of cells that has a role in regulating the immune system's response to the cells of the human body by down-regulating the immune system and promoting self tolerance by suppressing T cell inflammatory activity. |
| Recombinant Biotinylated Human PD-1/PDCD1 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Leu25-Gln167. |
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| PD-1/PDCD1 |
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| Human |
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| Q15116-1 |
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| Leu25-Gln167 |
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| The protein has a predicted MW of 18.9 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on the Tris-Bis PAGE result. |
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| Immobilized Biotinylated Human PD-1, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Human PD-L1, hFc Tag with the EC50 of 73.1ng/ml determined by ELISA (QC Test). The affinity constant of 2.356 uM as determined in SPR assay (Biacore T200). See testing image for detail. |
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| C-His-Avi |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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